Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

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中文名稱：

人磷酸甘油酸變位酶2(PGAM2)酶聯免疫試劑盒
貨號：

CSB-E11282h
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人磷酸甘油酸變位酶2(PGAM2)酶聯免疫試劑盒（CSB-E11282h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PGAM2含量。PGAM2是糖酵解途徑中的關鍵酶，與心肌肥厚和心力衰竭相關。研究顯示，PGAM2在心肌肥厚向心衰進展中起重要作用，其詳細功能尚不明確，可能通過調節能量代謝和氧化應激等途徑影響心臟功能。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本試劑盒適用于基礎科研領域，如研究骨骼肌代謝機制、紅細胞能量代謝動態、代謝相關疾病動物模型中的PGAM2表達水平變化，以及體外培養細胞中糖酵解途徑的調控分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

BPG dependent PGAM 2 ELISA Kit; BPG-dependent PGAM 2 ELISA Kit; GSD10 ELISA Kit; MGC88743 ELISA Kit; Muscle specific phosphoglycerate mutase ELISA Kit; Muscle-specific phosphoglycerate mutase ELISA Kit; OTTHUMP00000207787 ELISA Kit; PGAM 2 ELISA Kit; PGAM M ELISA Kit; PGAM-M ELISA Kit; Pgam2 ELISA Kit; PGAM2_HUMAN ELISA Kit; PGAMM ELISA Kit; phosphoglycerate mutase 2 (muscle) ELISA Kit; Phosphoglycerate mutase 2 ELISA Kit; Phosphoglycerate mutase isozyme M ELISA Kit
縮寫：

PGAM2
Uniprot No.：

P15259
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-94
1:2	Average %	102
	Range %	98-105
1:4	Average %	95
	Range %	89-99
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of human PGAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	98	92-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.526	2.417	2.472	2.327
500	1.608	1.505	1.557	1.412
250	0.884	0.855	0.870	0.725
125	0.486	0.494	0.490	0.345
62.5	0.329	0.306	0.318	0.173
31.2	0.265	0.257	0.261	0.116
15.6	0.197	0.189	0.193	0.048
0	0.145	0.144	0.145

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Proteomic analysis of effluents from perfused human heart for transplantation: identification of potential biomarkers for ischemic heart damage /,Proteome Science,2012

功能：

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
基因功能參考文獻：
1. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth. PMID: 24786789
相關疾病：

Glycogen storage disease 10 (GSD10)
蛋白家族：

Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
組織特異性：

Expressed in the heart and muscle. Not found in the liver and brain.
數據庫鏈接：

HGNC: 8889

OMIM: 261670

KEGG: hsa:5224

STRING: 9606.ENSP00000297283

UniGene: Hs.632642

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.