Human irisin ELISA kit

文獻引用 (43) 說明書下載

中文名稱：

人鳶尾素酶聯免疫試劑盒
貨號：

CSB-EQ027943HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人鳶尾素酶聯免疫試劑盒（CSB-EQ027943HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的irisin含量。Irisin是一種由運動誘導產生的肌肉因子。背景上，它被視為運動益處的重要介質。研究機制方面，Irisin能促進白色脂肪棕色化，增加能量消耗，還參與調節葡萄糖代謝、骨代謝等過程，在代謝性疾病等領域有潛在治療價值。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，科研人員可將其應用于運動生理學、代謝調控機制、脂肪組織功能研究等領域，通過定量分析不同干預條件下（如運動訓練、藥物處理）樣本中鳶尾素濃度變化，為探索代謝相關疾病的分子機制提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

irisin
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

84-96

1:2

Average %

Range %

91-103

1:4

Average %

103

Range %

97-109

1:8

Average %

Range %

83-95

回收率：

The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	94-106
EDTA plasma (n=4)	106	101-112

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.690	2.752	2.721	2.616
100	2.402	2.371	2.387	2.282
50	1.965	1.853	1.909	1.804
25	1.355	1.247	1.301	1.196
12.5	0.764	0.735	0.750	0.645
6.25	0.495	0.464	0.480	0.375
3.12	0.251	0.266	0.259	0.154
0	0.106	0.103	0.105

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Correlation of serum irisin levels with diabetic nephropathy: an exhaustive systematic appraisal and meta-analytical investigation Y Deng, Y Shen, Y Wu, M Wen, F Wang,Frontiers in Endocrinology,2025
Physical exercise modulates plasma irisin levels in endurance athletes: Implications for Parkinson's disease P Grillo, D Di Martino, A Calculli, R De Icco,Neurobiology of Disease,2025
The Influence of Irisin on Selected Organs—The Liver, Kidneys, and Lungs: The Role of Physical Exercise M Cia?owicz, M Wo?niewski, E Murawska-Cia?owicz,Cells,2025
Examining changes in serum levels of Irisin and Brain-Derived Neurotrophic Factor in inactive middle-aged men in two phases of training and detraining R Afshar, M Rahmati,Journal of Ilam University of Medical Sciences,2025
Dissociation of plasma oxyntomodulin levels from anthropometric measures and metabolic markers in women with polycystic ovary syndrome RC Damasceno, FR Oliveira,Archives of endocrinology and metabolism,2025
High Serum Levels of Irisin, Visfatin and Adiponectin as Potential Independent Risk Factors for Diabetic Nephropathy Progression in Patients With Type 2 Diabetes Mellitus BH Elesawy, A El Askary, OM Mehanna, EA Elmorsy,In Vivo,2025
Curcumin supplementation combined with high intensity interval training modulates serum irisin and lipid profile in obese women: "A randomized double-blind clinical trial" BJ Gharakhanlou, SB Bonab, A Amaghani,Contemporary Clinical Trials Communications,2025
Acute high-intensity interval exercise is superior to moderate-intensity continuous exercise in enhancing endothelial function and its associated biomarkers in sedentary young individuals: the possible involvement of lactate Z Liu, J Huang, M Hu, X Cui, L Leng, K Wang,Journal of Exercise Science & Fitness,2024
The impact of six-week dihydrogen-pyrroloquinoline quinone supplementation on mitochondrial biomarkers, brain metabolism, and cognition in elderly individuals with mild cognitive impairment: a randomized controlled trial S Baltic,Journal of Nutrition Health & Aging,2024
????? ??? ???? ????? ?????? ???? ?? ???????? ??????????? ?? ??????? ??????? ? ???? ???????? ?????? ? ????? ????? ???????? ???????,/,/
Elevated serum irisin levels in boys with central precocious puberty independent of BMI D Zeng,Endokrynologia Polska,2024
Serum Irisin in Polycystic Ovary Syndrome and its Alteration with Metformin Intervention R Telagareddy,/,2024
Interleukin-15 Contributes to the Benefits of Βeta-Alanine Supplementation on the Performance of Elite Volleyball Players YR Qanbar,/,2024
Comparative effects of different types of exercise on endothelial function, arterial stiffness, and executive function in sedentary young individuals: a randomized controlled trial J Huang,/,2024
Serum irisin levels increase in girls with central precocious puberty not dependent on BMI: a pilot study Y Chen,Endocrine connections,2023
The relationship between sarcopenia detected in newly diagnosed colorectal cancer patients and FGF21, irisin and CRP levels U Oflazoglu,European geriatric medicine,2023
Relationship among Body Composition, Adipocytokines, and Irisin on Exercise Capacity and Quality of Life in COPD: A Pilot Study G Cuttitta,Biomolecules,2023
HFpEF and Atrial Fibrillation: The Enigmatic Interplay of Dysmetabolism, Biomarkers, and Vascular Endothelial Dysfunction J Bosanac,Disease markers,2022
Effects of functional training with blood occlusion on the irisin, follistatin, and myostatin myokines in elderly men F Pazokian,European Review of Aging and Physical Activity,2022
The effects of different types of exercise on circulating irisin levels in healthy individuals and in people with overweight, metabolic syndrome and type 2 diabetes SG Parada-Sánchez,Physiological research,2022
Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB and lysosomal dysfunction in dairy cows with clinical ketosis ZhiyuanFang,Journal of Dairy Science,2022
Irisin levels are correlated with inflammatory markers in frontotemporal dementia VG Fraga,Journal of Clinical Neuroscience,2021
Emerging evidence for the opposite role of circulating irisin levels and brown adipose tissue activity measured by infrared thermography in anthropometric and metabolic profile during childhood F De Meneck,Journal of Thermal Biology,2021
Analysis of changes in serum levels and gene expression profiles of novel adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and their correlation with serum C-reactive protein levels in women diagnosed with endometriosis E Kaya-Sezginer,/,2021
Effect of Chronic Resistance Training on Circulating Irisin: Systematic Review and Meta-Analysis of Randomized Controlled Trials Pedro L. Cosio,International Journal of Environmental Research and Public Health,2021
Effects of Interval Jump Rope Exercise Combined with Dark Chocolate Supplementation on Inflammatory Adipokine, Cytokine Concentrations, and Body Composition in Obese Adolescent Boys M Eskandari,Nutrients,2020
Irisin level and neonatal birthweight: A systematic review and meta-analysis D Shan,European Journal of Obstetrics & Gynecology and Reproductive Biology,2020
Several Circulating Biomarkers for PCOS Diagnosis Luo P, et al,Experimental and Clinical Endocrinology & Diabetes,2019
Comprehensive evaluation of irisin levels in fetomaternal circulation of pregnant women with obesity or gestational diabetes mellitus Ali Seven,Irish Journal of Medical Science,2019
Alpha lipoic acid protects against dexamethasone-induced metabolic abnormalities via APPL1 and PGC-1 α up regulation Mohammed MA, et al,Steroids,2019
The effect of hypothyroidism on serum irisin level in patients with nondiabetic chronic kidney disease: A pilot study with a cross-sectional design Milind Machhindra Patil.et al,Saudi Journal of Kidney Diseases and Transplantation,2018
Abnormal irisin level in serum and endometrium is associated with metabolic dysfunction in polycystic ovary syndrome patients Wei Wang.et al,CLIN ENDOCRINOL,2018
The association between circulating irisin levels and different phenotypes of polycystic ovary syndrome L. Zhang.et al,Journal of Endocrinological Investigation,2018
High Irisin Levels in Overweight/Obese Children and Its Positive Correlation with Metabolic Profile, Blood Pressure and Endothelial Progenitor Cells F De Meneck.et al,/,2018
Irisin and Visfatin Predicts Severity of Diabetic Nephropathy Ramalingam Mageswari.et al,Indian Journal of Clinical Biochemistry,2018
The combination of exercise training and Zataria multiflora supplementation increase serum irisin levels in postmenopausal women Ghanbari-Niaki, Abbass.et al,Integrative Medicine Research,2018
SERUM IRISIN AND LEPTIN LEVELS IN OBESE AND NON-OBESE WOMEN WITH POLYCYSTIC OVARY SYNDROME WITH REFERENCE TO GLUCOSE SHATHA H. ALI.et al,International Journal of Pharmacy and Pharmaceutical Sciences ,2017
Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review Dinas PC.et al,?F1000Res,2017
Physiology and role of irisin in glucose homeostasis Nikolaos Perakakis Georgios A.et al,Nature Reviews Endocrinology,2017
Free androgen index and Irisin in polycystic ovary syndrome Li H. et al,J Endocrinol Invest,2015
Decreased saliva/serum irisin concentrations in the acute myocardial infarction promising for being a new candidate biomarker for diagnosis of this pathology. Aydin S.et al,Peptides.,2014
Irisin levels before and after physical activity among school-age children with different BMI: A direct relation with leptin Palacios-González B et al,Obesity,2015
Elevated Circulating Levels of Irisin and the Effect of Metformin Treatment in Women with Polycystic Ovary Syndrome Li M et al,J Clin Endocrinol Metab,2015

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最新研究進展：

Irisin是一種由肌肉細胞產生的激素，在身體代謝調節中發揮著重要作用。最近的研究表明，Irisin對改善糖尿病、肥胖和心血管疾病等慢性病的預防和治療具有潛在價值。此外，研究人員正在探索Irisin對其他疾病的影響，如阿爾茨海默病、肌肉萎縮和骨質疏松癥等。雖然Irisin的作用機制還沒有完全理解，但這種激素已經成為新的藥物開發領域的熱門研究方向之一。

Human irisin ELISA kit

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