Human interferon-inducible protein 10,IP-10 ELISA Kit

Human interferon-inducible protein 10,IP-10 ELISA Kit

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中文名稱：

人干擾素誘導蛋白10(IP-10/CXCL10)酶聯免疫試劑盒
貨號：

CSB-E08181h
規格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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產品描述：

人干擾素誘導蛋白10(IP-10/CXCL10)酶聯免疫試劑盒（CSB-E08181h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL10含量。CXCL10屬于趨化因子，在炎癥、免疫反應等生理病理過程中發揮重要作用。研究顯示它可與CXCR3受體結合，調控免疫細胞趨化與活化，在腫瘤、自身免疫病等疾病中異常表達，成為潛在治療靶點，圍繞其開展的干預研究具有重要意義。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，靈敏度為7.8 pg/mL。適用于基礎科研中探索炎癥相關信號通路、評估疾病模型免疫狀態、分析組織微環境細胞因子網絡等場景，為免疫調控機制研究、藥物干預效果評估及生物標志物篩選提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Interferon gamma induced factor MOB1; mouse; homolog of ELISA Kit; Interferon gamma induced protein 10 ELISA Kit; 10 kDa interferon gamma induced protein ELISA Kit; 10 kDa interferon gamma-induced protein ELISA Kit; C X C motif chemokine 10 ELISA Kit; C7 ELISA Kit; Chemokine (C X C motif) ligand 10 ELISA Kit; Chemokine CXC motif ligand 10 ELISA Kit; Crg 2 ELISA Kit; CRG2 ELISA Kit; CXCL10 ELISA Kit; CXCL10(1-73) ELISA Kit; CXL10_HUMAN ELISA Kit; Gamma IP10 ELISA Kit; Gamma-IP10 ELISA Kit; gIP 10 ELISA Kit; GIP10 ELISA Kit; IFI10 ELISA Kit; INP 10 ELISA Kit; INP10 ELISA Kit; Interferon activated gene 10 ELISA Kit; Interferon activated gene 10 ELISA Kit; Interferon gamma induced cell line ELISA Kit; Interferon inducible cytokine IP 10 ELISA Kit; Interferon inducible cytokine IP10 ELISA Kit; IP 10 ELISA Kit; IP-10 ELISA Kit; Mob 1 ELISA Kit; MOB1 ELISA Kit; Protein 10 from interferon (gamma) induced cell line ELISA Kit; SCYB10 ELISA Kit; Small inducible cytokine B10 ELISA Kit; Small inducible cytokine B10 precursor ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member 10 ELISA Kit; Small inducible cytokine subfamily B CXC member 10 ELISA Kit; Small inducible cytokine subfamily B; member 10 ELISA Kit; Small-inducible cytokine B10 ELISA Kit
縮寫：

CXCL10
Uniprot No.：

P02778
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	98
	Range %	91-105
1:4	Average %	96
	Range %	92-103
1:8	Average %	92
	Range %	84-98

回收率：

The recovery of human IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	93	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.923	2.963	2.943	2.832
1000	2.151	2.176	2.164	2.053
500	1.255	1.276	1.266	1.155
250	0.706	0.671	0.689	0.578
125	0.434	0.399	0.417	0.306
62.5	0.299	0.285	0.292	0.181
31.25	0.248	0.253	0.251	0.140
0	0.110	0.111	0.111

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The role of cyclic di-GMP in biomaterial-associated infections caused by commensal Escherichia coli S Li, C Bi, B Xiang, Z Wang, H Yang, C Fu, L Chen,Plos one,2025
Renal remodeling by CXCL10-CXCR3 axis-recruited mesenchymal stem cells and subsequent IL4I1 secretion in lupus nephritis Q Zhang, Y Shan, L Shen, Q Ni, D Wang, X Wen, H Xu, X Liu, Z Zeng, J Yang, Y Wang, J Liu,Signal Transduction and Targeted Therapy,2024
Endosialin-positive CAFs promote hepatocellular carcinoma progression by suppressing CD8+ T cell infiltration L Gan, T Lu, Y Lu, H Song, J Zhang, K Zhang,Journal for ImmunoTherapy of Cancer,2024
Neutrophil extracellular trap-induced intermediate monocytes trigger macrophage activation syndrome in adult-onset Still's disease J Jia,BMC medicine,2023
Dipeptidyl peptidase 4 as a potential serum biomarker for disease activity and treatment response in rheumatoid arthritis J Yu,International immunopharmacology,2023
The Efficacy and Psychoneuroimmunology Mechanism of Camouflage Combined With Psychotherapy in Vitiligo Treatment Y Chang,Frontiers in medicine,2022
MiR-18a-downregulated RORA inhibits the proliferation and tumorigenesis of glioma using the TNF-α-mediated NF-κB signaling pathway Jiang Y,EBioMedicine,2020
Up-regulation of Interferon-inducible protein 16 contributes to psoriasis by modulating chemokine production in keratinocytes Cao T.et al,Sci Rep.,2016

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平均分:

5.0分 - 1 個評價

樣品類型：血漿（抗凝劑） IP-10

樣品信息：人

稀釋比：沒有稀釋

產品評價：我們實驗室絕大多數試劑盒都來自華美生物CUSABIO，發貨速度快，性能穩定，使用CSB-E08181h分別檢測了實驗和對照個體血漿中的干擾素-γ-誘導蛋白 10 (IP-10) 的水平，結果顯示在實驗個體中IP-10的含量有所增加，與預期的結果一致，能較好地完成實驗計劃，節省實驗時間。而且價格也比較實惠，同一個課題組使用的不少，采購也方便。

By 王老師

最新研究進展：

CXCL10，也稱為干擾素γ誘導蛋白-10（IP-10），是一種細胞因子，參與炎癥和免疫反應。最新研究表明CXCL10在腫瘤免疫治療中具有重要作用，它可以增強腫瘤細胞的免疫原性，并吸引和激活具有殺傷作用的T細胞。
功能：

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.
基因功能參考文獻：
1. results of study suggest that the CXCL10 overexpression in the salivary glands is caused mainly by IFN-gamma-stimulated salivary gland ductal cells. PMID: 29549479
2. Urinary CXCL10 reflects subclinical inflammation within the tubulointerstitial and peritubular capillary spaces, but not the vascular/systemic compartments PMID: 28902772
3. CXCL10 in the serum of patients with vitiligo and alopecia areata was significantly elevated compared with healthy controls, but was similar between patients with vitiligo and AA PMID: 27863059
4. IP-10 might be involved in the pathogenesis of thrombocytopenia (T), anasarca (A), fever (F), reticulin fibrosis (R), and organomegaly (O) -idiopathic Multicentric Castleman disease. PMID: 28205564
5. CXCL10, not CXCL9 or CXCL11, induced IL-9 expression in the liver tissue. PMID: 29860220
6. serum IP-10 level and the IFN-gamma/IL-4 ratio have great potential to predict significant fibrosis among chronic hepatitis B patients. PMID: 28067328
7. No association between IP-10 serum-levels and cancer-related fatigue. PMID: 30188739
8. CXCL10 rs1439490 G/G is positively associated with seronegative occult hepatitis C virus infection and antiviral treatment outcome. PMID: 29853737
9. Age-related epigenetic and genetic factors which contribute to the dysregulation of CXCL10. PMID: 29223680
10. COMMD7 activates CXCL10 production by regulating NFkappaB and the production of ROS. The present study highlighted the role of COMMD7 in the development of HCC, and provides novel options for anticancer drug design. PMID: 29532873
11. Nab-PTX treatment could increase CXCL10 expression. PMID: 29902349
12. The frequency of non-classical monocytes spontaneously producing CXCL10 was increased in both limited and diffuse systemic sclerosis PMID: 29127442
13. serum levels in active vitiligo significantly elevated compared to those in stable vitiligo patients PMID: 29115683
14. Plasma CXCL10 levels are significantly higher in SPs pre-HAART and RPs pre-HAART when compared with HIV-negative controls. PMID: 29122683
15. Hepatitis b vaccine was efficient in enhancing IP-10 level with HBsAg clearance, or reduction to a favorable level. PMID: 28802168
16. Results show that the expression of IP-10 in peripheral blood of patients with HBV-associated acute-on-chronic liver failure (HBV-ACLF) was significantly high and correlated with the severity of liver failure. IP-10 played an important role in the pathogenesis and progression of HBV-ACLF. PMID: 29058291
17. Differential regulation of cytokine release at both transcriptional and post-transcriptional levels, suppresses type-I-IFN induction yet allows for CXCL10 secretion during imDNA-induced cellular stress. PMID: 27941826
18. the elevated concentrations of CXCL13, CXCL8, and CXCL10 or their increasing CSF/serum ratios may be potential biomarkers of neurosyphilis PMID: 27650493
19. Results highlight the role of phytohaemagglutin-stimulated peripheral blood mononuclear cells from patients with Alzheimer's disease on the expression of chemokines CXCL10 and CCL4 by endothelial cells and H4 cell line (mimicking the brain parenchyma) in a human blood brain barrier model. PMID: 28413983
20. viral and bacterial co-infection modulates the JAK-STAT signaling pathway and leads to exacerbated IP-10 expression, which could play a major role in the pathogenesis of pneumonia. PMID: 27922126
21. Study suggests that rs56061981 and rs56216945 in CXCL10 gene promoter contribute COPD susceptibility. PMID: 29285564
22. Our findings showed that the assessment of serum IP-10 level could be a predictive factor for SVR and it was associated with fibrosis, necroinflammatory activity, significant steatosis and IR in patients with chronic HCV infection. PMID: 28862188
23. In conclusion, IL-29 enhanced CXCL10 production in human oral epithelial cells via the p38 MAPK, STAT3, and NF-kappaB pathways, which might control Th1-cell accumulation in periodontal lesions and be involved in pathological processes in periodontal disease. PMID: 28753407
24. Interferon-gamma-inducible protein 10 (IP-10) directly promoted hepatocyte apoptosis, and baseline IP-10 levels may predict the decrease in the hepatitis B surface antigen levels after entecavir therapy in patients with chronic hepatitis B. PMID: 28614914
25. data indicate that IP-10 is associated with disease activity and perseverance of rheumatoid arthritis. PMID: 28592626
26. CXCL10 in addition to IFN-gamma can be used to differentiate among Mycobacterium tuberculosis infection possibilities. PMID: 28610785
27. Increased amniotic fluid CXCL10 concentration is associated with chronic chorioamnionitis or maternal anti-fetal rejection, whereas increased amniotic fluid IL-6 concentration is associated with acute histologic chorioamnionitis. PMID: 28544362
28. Cord blood CXCL10 levels were negatively associated with mite sensitization at age 3. A high cord blood CCL22/CXCL10 chemokine ratio was significantly associated with a higher risk of allergic sensitization at age 3. PMID: 27863395
29. CXCL10/IP-10 transcript showed up to 20 fold-increase, with similar changes detectable at the protein level in melanoma cells overexpressing PDFGRA. PMID: 27764787
30. this study suggests that GG genotype of CXCL10 -135G/A (rs56061981) polymorphism decreased CXCL10 expression in T cells which may have defective recruitment of mononuclear cells at the site of infection as well granuloma formation and in turn contribute to progression of tuberculosis PMID: 28336310
31. The regular early post-transplantation monitoring of urinary miR-155-5p and CXCL10 can help in the prognosis of acute rejection and graft dysfunction after kidney transplantation. PMID: 28880456
32. gene polymorphisms of IL-8(-251T/A) and IP-10(-1596C/T) are associated with susceptibility and progression of type 2 diabetic retinopathy in a northern Chinese population PMID: 27935598
33. Higher serum levels of CXCL10 are found in patients with non-segmental vitiligo (NSV) and NSV + autoimmune thyroiditis than in controls. PMID: 28698095
34. results indicated that CXCL10, a pro-inflammatory chemokine, might be involved in the abnormal immune response in aplastic anaemia. PMID: 28411045
35. IP-10 low in the tumor microenvironment can be used as potential indicators for the progression of non-small cell lung cancer PMID: 28375674
36. The serum IP10 concentrations increase in women with polycystic ovary syndrome (PCOS), moreover, IP10 appears to be correlated with the inflammatory and Insulin resistance statuses of PCOS. IP10 may be a potential biomarker to estimate the disease activity of PCOS. PMID: 28051885
37. EGF and IP-10 were significantly elevated and GRO levels were lower in the tear profile of HIV patients with dry eye disease (DED) compared to immunocompetent patients with DED. PMID: 27585367
38. Determination of serum IP-10 levels before treatment could be useful for predicting favorable virologic response to TVR-based triple therapy, especially in patients with IL28B non-TT genotype. PMID: 27541605
39. In lipotoxic hepatocytes, MLK3 activates a MAPK signaling cascade, resulting in the activating phosphorylation of STAT1, and CXCL10 transcriptional upregulation. PMID: 28262979
40. Neuroendocrine-like cells promote the chemotaxis activity of tumor-associated macrophages (TAM) via CXCL10 and CXCL11. PMID: 27034164
41. These results showed that the higher levels of CXCL10, CCL20 and CCL22 were associated with ischemic heart disease. The serum levels of chemokines may influence by the certain traditional risk factors of IHD and some studied SNPs, but did not influence by treatment and gender of patients. PMID: 27152707
42. High CXCL10 expression is associated with clear-cell renal cell carcinoma. PMID: 26910919
43. a massive and selective serum CXCL10 response in R. conorii-infected patients, likely reflecting release from infected endothelial cells characterized by infiltrating T cells and monocytes. PMID: 27180202
44. The strongest OR was for CXCL8 (interleukin-8) in serum (96.8, 95% CI: 11.9-790.2). Of these 15 markers, 6 were also significantly elevated in serum from Chile (CCL20, C-reactive protein, CXCL8, CXCL10, resistin, serum amyloid A). PMID: 27173614
45. These findings elucidate an NFAT-MAPK signaling paradigm for induction of isletokine expression in beta-cells and reveal IP-10 as a primary therapeutic target to prevent beta-cell-induced inflammatory loss of graft function after islet cell transplantation. PMID: 28855240
46. High urine CXCL10 level is associated with acute rejection in Histologically Stable Kidney transplant Recipients. PMID: 26694099
47. IL-10, IL-17 and IP-10 response to tubercular antigens act as potent discriminative markers for active tuberculosis among pulmonary tuberculosis suspects. PMID: 27450011
48. The performance of IFNG release assays is robust despite variations in the incubation temperature between 37 degrees C and 39 degrees C for the diagnosis of latent tuberculosis infection. PMID: 27156612
49. A novel function of CXCL10 in mediating monocyte production of proinflammatory cytokines in inflammatory bowel diseases has been described. PMID: 28899907
50. CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043
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亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
組織特異性：

Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation.
數據庫鏈接：

HGNC: 10637

OMIM: 147310

KEGG: hsa:3627

STRING: 9606.ENSP00000305651

UniGene: Hs.632586

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.