Human intercellular adhesion molecule 1,ICAM-1 ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人細(xì)胞間粘附分子1(ICAM-1/CD54)酶聯(lián)免疫試劑盒（CSB-E04574h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ICAM1含量。ICAM1即細(xì)胞間黏附分子-1，其在免疫應(yīng)答、炎癥反應(yīng)等生理病理過程中發(fā)揮重要作用。研究機(jī)制方面，它可介導(dǎo)細(xì)胞間黏附與信號傳遞。異常表達(dá)與炎癥性疾病、腫瘤轉(zhuǎn)移等密切相關(guān)，因此是開發(fā)相關(guān)治療藥物的潛在靶點(diǎn)。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，靈敏度為0.195 ng/mL。該產(chǎn)品可為炎癥反應(yīng)機(jī)制研究、免疫調(diào)節(jié)藥物開發(fā)、心血管疾病模型建立等體外實(shí)驗(yàn)提供可靠數(shù)據(jù)支持，尤其適用于體外模擬炎癥條件下內(nèi)皮細(xì)胞活化評估、免疫細(xì)胞互作研究以及疾病相關(guān)生物標(biāo)志物檢測等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
Antigen identified by monoclonal ELISA Kit BB2 ELISA Kit; BB 2 ELISA Kit; BB2 ELISA Kit; CD 54 ELISA Kit; CD_antigen=CD54 ELISA Kit; CD54 ELISA Kit; Cell surface glycoprotein P3.58 ELISA Kit; Human rhinovirus receptor ELISA Kit; ICAM 1 ELISA Kit; ICAM-1 ELISA Kit; ICAM1 ELISA Kit; ICAM1_HUMAN ELISA Kit; intercellular adhesion molecule 1 (CD54) ELISA Kit; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor ELISA Kit; Intercellular adhesion molecule 1 ELISA Kit; Major group rhinovirus receptor ELISA Kit; MALA 2 ELISA Kit; MALA2 ELISA Kit; MyD 10 ELISA Kit; MyD10 ELISA Kit; P3.58 ELISA Kit; Surface antigen of activated B cells ELISA Kit; Surface antigen of activated B cells, BB2 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
0.78 ng/mL-50 ng/mL
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靈敏度：
0.195 ng/mL
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Immunology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) | |
1:10 | Average % | 84 | |
Range % | 80-92 | |
1:20 | Average % | 94 | |
Range % | 91-102 | |
1:40 | Average % | 95 | |
Range % | 90-99 | |
1:80 | Average % | 95 | |
Range % | 86-99 | |
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回收率：
The recovery of human ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 96 | 91-103 | |
EDTA plasma (n=4) | 93 | 90-102 | |
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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ng/ml | OD1 | OD2 | Average | Corrected | |
50 | 2.438 | 2.604 | 2.521 | 2.362 | |
25 | 1.986 | 1.897 | 1.941 | 1.782 | |
12.5 | 1.408 | 1.358 | 1.383 | 1.224 | |
6.25 | 0.913 | 0.929 | 0.921 | 0.762 | |
3.12 | 0.470 | 0.451 | 0.460 | 0.301 | |
1.56 | 0.341 | 0.353 | 0.347 | 0.188 | |
0.78 | 0.259 | 0.274 | 0.266 | 0.107 | |
0 | 0.163 | 0.155 | 0.159 | | |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ICAM1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ICAM1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Oral Administration of Sodium Nitrate to Metabolic Syndrome Patients Attenuates Mild Inflammatory and Oxidative Responses to Acute Exercise
X Capó,Antioxidants (Basel),2020
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KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke
Zhanga X, et al,/,2019
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Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs
Esplugas R, et al,Anticancer Research,2019
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Expression levels of aldose reductase enzyme, vascular endothelial growth factor, and intercellular adhesion molecule-1 in the anterior lens capsule of diabetic cataract patients
Bessa AS.et al,journal of cataract and refractive surgery,2018
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Effect of the Diabetic Environment On the Expression of MiRNAs in Endothelial Cells: Mir-149-5p Restoration Ameliorates the High Glucose-Induced Expression of TNF-α and ER Stress Markers
Yuan J.et al,Cell Physiol Biochem.,2017
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MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression
Deng H.et al,Cell Physiol Biochem.,2017
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Chemical constituents and sources of ambient particulate air pollution and biomarkers of endothelial function in a panel of healthy adults in Beijing, China.
Wu S.et al,Sci Total Environ.,2016
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Evaluation of Endothelial Cell Adhesion Molecules and Anti-C1q Antibody in Discriminating between Active and Non-Active Systemic Lupus Erythematosus
Mahayidin H.et al,Malays J Med Sci.,2016
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Omentin inhibits TNF-a-induced expression of adhesion molecules in endothelial cells via ERK/NF-kB pathway
Xia Zhong et al,Biochemical and Biophysical Research Communications,2012
靶點(diǎn)詳情
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最新研究進(jìn)展：
ICAM1是一種細(xì)胞間黏附分子，它能夠調(diào)節(jié)免疫細(xì)胞的遷移和黏附。最近的研究表明，ICAM1還可能在多種疾病的發(fā)生和發(fā)展中發(fā)揮重要作用，包括心血管疾病、炎癥性疾病和腫瘤等。
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功能：
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.; (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.; (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.; (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
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基因功能參考文獻(xiàn)：
- miR-335-5p could target 3'UTR of ICAM-1 and inhibit its expression, preventing invasion and metastasis of thyroid cancer cells. PMID: 30119270
- AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-kappaB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases. PMID: 29193068
- Our meta-analyses provide no evidence of the association of ICAM-1 rs5498 with diabetic retinopathy in type 2 diabetic patients. PMID: 30419874
- CORM-2 inhibits P. aeruginosa-induced PGE2/IL-6/ICAM-1 expression and lung inflammatory responses by reducing the Reactive Oxygen Species generation and the inflammatory pathways. PMID: 30007888
- Smooth muscle cell-derived bioactive soluble factors may stimulate the ICAM-1 expression of cocultured endothelial cells, possibly leading to leukocyte migration into the subendothelial space. PMID: 29852173
- These results together with the authors' previous study, have identified that CNOT1 provides a platform for the recruitment of TTP and CNOT7, and is involved in TTPmediated ICAM1 and IL8 mRNA decay. PMID: 29956766
- ICAM-1 expression is not significantly linked to metastatic disease in pancreatic ductal adenocarcinoma. PMID: 29355490
- In conclusion, this meta-analysis indicates that ICAM-1 gene rs5498 polymorphism decreases the risk of CAD PMID: 30290609
- Data suggest that serum levels of soluble ICAM1 are higher in young adults with reduced physical activity as compared to young adults who participate in optimal physical activity. This study was conducted in Bulgaria with medical and dental students aged 20 +/-2 years. PMID: 29183155
- TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
- not a specific screening marker for pulmonary arterial hypertension in systemic sclerosis PMID: 29687288
- The ICAM-1 expression level determines the susceptibility of human endothelial cells to simulated microgravity. PMID: 29080356
- the combination of IL-6 -572C/G and ICAM-1 K469E polymorphisms have a synergistic effect on the onset of Sudden sensorineural hearing loss. PMID: 29695657
- Peripheral blood lymphocyte subsets in patients with lung cancer are different from those in healthy people, and circulating CD44+ and CD54+ lymphocytes seem to be a promising criterion to predict survival in lung cancer patients undergoing chemotherapy PMID: 29148014
- serum ICAM-1 levels were associated with type 2 diabetes mellitus with microalbuminuria leading to severity of diabetic kidney disease. PMID: 29310968
- Serum CCL2 and sICAM-1 concentrations were significantly decreased in CNS tumors in comparison with the comparative group. Among proteins tested in the serum, a higher area under the ROC curve (AUC) revealed CCL2 compared to sICAM-1 in differentiating subjects with CNS brain tumors from non-tumoral subjects. PMID: 29086194
- Patient-derived ATC cells overexpressed ICAM-1 and were largely eliminated by autologous ICAM-1 CAR T cells in vitro and in animal models. Our findings are the first demonstration of CAR T therapy against both a metastatic, thyroid cancer cell line and advanced ATC patient-derived tumors that exhibit dramatic therapeutic efficacy and survival benefit in animal studies PMID: 29025766
- Data indicate ICAM-1 as an essential receptor for both acute hemorrhagic conjunctivitis (AHC)-causing and non-AHC strains. PMID: 29284752
- The results of cell adhesion and western blotting assays demonstrated that arachidin-1 attenuated tumor necrosis factor (TNF)-alpha-induced monocyte/EC adhesion and intercellular adhesion molecule-1 (ICAM-1) expression PMID: 29115410
- anthropometric and physiological parameters do not affect the response of ICAM-1 to exercise in healthy men. PMID: 29696063
- 15-LOX-1 expression in colon and prostate cancer cells leads to reduced angiogenesis. These changes could be mediated by an increase in the expression of both ICAM-1 and the anti-angiogenic protein TSP-1. PMID: 28757355
- Single nucleotide polymorphisms in ICAM1 (rs1799969) and SERPINB2 (rs6103) genes were found to be protective against thalidomide-induced peripheral neuropathy (TiPN). In children with inflammatory bowel disease, TiPN is common but mild and generally reversible. Cumulative dose seems to be the most relevant risk factor, whereas polymorphisms in genes involved in neuronal inflammation may be protective. PMID: 28817461
- analysis of aberrant DNA methylation and hydroxymethylation of the ICAM1 gene promoter in the thyrocytes of Autoimmune Thyroiditis patients PMID: 28388873
- Authors demonstrate that the membrane-bound ICAM-1 isoform is necessary and sufficient to promote inflammation-dependent extracellular matrix contraction, which favors cancer cell invasion. Indeed, ICAM-1 mediates generation of acto-myosin contractility downstream of the Src kinases in stromal fibroblasts. PMID: 27901489
- Thrombin activated platelet releasing exosomes convey miRNA between cells. miRNA-223 regulates the expression of molecules adhesion including ICAM-1. miRNA-223 downregulated ICAM-1 mainly by impacting NF-kappaB and the MAPK pathway. PMID: 28460288
- Data suggest that obese children/adolescents have increased circulating biomarkers of endothelial dysfunction (here, ICAM1) and early signs of renal damage, similarly to children/adolescents with type 1 diabetes (T1D), confirming obesity to be a cardiovascular risk factor as is T1D. PMID: 27246625
- ICAM-1 (and IL-17) polymorphisms showed significant association with Guillian-Barre syndrome. PMID: 27595159
- Airway ICAM-1 expression is markedly upregulated in CAL group, which could be crucial in rhinoviral and NTHi infections. The parenchymal ICAM-1 is affected by smoking, with no further enhancement in CAL subjects. PMID: 28056984
- sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581
- Atorvastatin strengthens Skp2 binding to FOXO1 or ICAM1, leading to ubiquitination and degradation. Skp2-dependent ubiquitination of major pathogenic molecules is the key mechanism for statin's protective effect on endothelial function in diabetes. PMID: 28802579
- Augmented expression of endothelial adhesion molecules ICAM1/VCAM1 is involved in the pathophysiology of patients with antiphospholipid syndrome. PMID: 29096830
- CD133(+) CD44(+) CD54(+) cellular subpopulation of circulating tumor cells has a prognostic value in colorectal cancer patients with liver metastasis, especially in the survival of CRC patients with liver metastasis who did not undergo surgical treatment for metastasis. PMID: 29105339
- Data suggest that the residue volume at phenylalanine (Phe) in alpha1-helix is critical for alpha(L)/beta(2) integrin (CD49a/CD18) activation and binding with soluble/immobilized ICAM1 (intercellular cell adhesion molecule 1). PMID: 29079572
- Elevated serum uric acid concentration is significantly associated with inflammation of maternal systemic vasculature as indicated by increased TNF-alpha and ICAM-1 expression in women with preeclampsia. PMID: 26511169
- associated with hypertension and stroke risk in women PMID: 27235695
- Data indicate that CDH11, ICAM1 and CLDN3 were overexpressed in tumors when compared to normal esophagus, normal gastric and non-dysplastic Barrett's. PMID: 27363029
- High levels of serum ICAM-1 was associated with the development of multiple organ failure . High levels of VCAM-1 was associated with both multiple organ failure and in-hospital mortality. PMID: 27701021
- matrix stiffness-dependent ICAM-1 clustering is an important regulator of vascular inflammation PMID: 27444067
- PD increased CKIP-1 and Nrf2 levels in the kidney tissues as well as improved the anti-oxidative effect and renal dysfunction of diabetic mice, which eventually reversed the up-regulation of FN and ICAM-1 PMID: 28286065
- PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). PMID: 27703032
- These results suggest that SHP-2-via association with ICAM-1-mediates ICAM-1-induced Src activation and modulates VE-cadherin switching association with ICAM-1 or actin, thereby negatively regulating neutrophil adhesion to endothelial cells and enhancing their transendothelial migration. PMID: 28701303
- Although there was no association between sICAM-1 levels and affective temperament scores, sICAM-1 was related to the state severity of manic symptoms. PMID: 27693464
- It is a pro-inflammatory protein. PMID: 28390825
- HPMCs are capable of inhibiting growth of gastrointestinal tumors in a mechanism involving the anti-adhesive capabilities of sICAM-1 PMID: 28323210
- Following transepithelial migration, neutrophils adhesion to ICAM-1 resulted in activation of Akt and beta-catenin signaling, increased epithelial-cell proliferation, and wound healing. PMID: 26732677
- P-Selectin and ICAM-1 have roles in mediating THP-1 monocyte adhesion PMID: 28262902
- Dissection of the molecular mechanism revealed that the p38-Notch1 axis was the main downstream signaling pathway in CD54-mediated regulation of cancer stem cells in prostate cancers. PMID: 28042317
- We show that knockdown of the expression of mcircRasGEF1B reduces LPS-induced ICAM-1 expression. Additionally, we demonstrate that mcircRasGEF1B regulates the stability of mature ICAM-1 mRNAs. PMID: 27362560
- his study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospirosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels. PMID: 28686648
- data suggest that CD2AP acts as a negative regulator of ICAM-1 clustering, which limits the formation of ICAM-1 adhesion complexes to prevent uncontrolled neutrophil adhesion and transcellular transmigration. PMID: 28484055
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亞細(xì)胞定位：
Membrane; Single-pass type I membrane protein.
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蛋白家族：
Immunoglobulin superfamily, ICAM family
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