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中文名稱：人間α-胰蛋白酶抑制因子重鏈H1(ITIH1)酶聯免疫試劑盒
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貨號：CSB-E13743h
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人間α-胰蛋白酶抑制因子重鏈H1(ITIH1)酶聯免疫試劑盒（CSB-E13743h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、尿液、組織勻漿樣本中的ITIH1含量。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，本試劑盒可應用于基礎醫學研究中ITIH1的生物學功能解析、炎癥相關疾病模型的分子機制探索，以及藥物干預對細胞外基質重塑影響的體外評價等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：H1P ELISA Kit; IATIH ELISA Kit; IGHEP1 ELISA Kit; Inter alpha (globulin) inhibitor H1 ELISA Kit; Inter alpha (globulin) inhibitor H1 polypeptide ELISA Kit; Inter alpha trypsin inhibitor heavy chain 1 ELISA Kit; Inter alpha trypsin inhibitor heavy chain H1 ELISA Kit; Inter-alpha-inhibitor heavy chain 1 ELISA Kit; Inter-alpha-trypsin inhibitor complex component III ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H1 ELISA Kit; ITI heavy chain H1 ELISA Kit; ITI-HC1 ELISA Kit; ITIH ELISA Kit; ITIH1 ELISA Kit; ITIH1_HUMAN ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
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縮寫：ITIH1
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, cell culture supernates, urine, tissue homogenates
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檢測范圍：15.6 ng/mL-1000 ng/mL
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靈敏度：3.9 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cell Biology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 86 Range % 80-92 1:200 Average % 97 Range % 91-105 1:400 Average % 99 Range % 92-110 1:800 Average % 97 Range % 86-98 -
回收率：
The recovery of human ITIH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-98 EDTA plasma (n=4) 97 90-104 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 1000 2.400 2.418 2.409 2.328 500 2.088 1.999 2.004 1.923 250 1.562 1.555 1.559 1.478 125 1.228 1.211 1.220 1.139 62.5 0.689 0.665 0.677 0.596 31.25 0.452 0.436 0.444 0.363 15.6 0.270 0.254 0.262 0.181 0 0.084 0.078 0.081 -
數據處理：
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貨期：3-5 working days
引用文獻
- Proteomic Analysis of Serum from Patients with Major Depressive Disorder to Compare Their Depressive and Remission Statuses Jiyeong Lee et al, Psychiatry Investig,2015
產品評價
相關產品
靶點詳情
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功能：May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.; Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
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基因功能參考文獻：
- Furthermore, thrombin treatment of the inflammatory matrix leads to dissolution of HC-HA cable structures and abolishes leukocyte adhesion. These data establish a novel mechanism whereby thrombin cleavage of HC1 regulates the adhesive properties of an inflammatory HA matrix. PMID: 27679489
- Data suggest that Mg2+ or Mn2+ (but not Ca2+) induce a conformational change in inter-alpha-inhibitor (ITIH1 and ITIH2) and a bikunin/chondroitin sulfate-dependent increase in thermodynamic stability; bikunin binds adjacent to the two heavy chains. PMID: 26728454
- The results of this study revealed that the shared genetic risk factors of both schizophrenia and major depressive disorder exist in ITIH family genes in the Han Chinese population. PMID: 24389398
- Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
- Human inter-alpha-inhibitor is a substrate for factor XIIIa and tissue transglutaminase. PMID: 21939789
- Transfection into H460M Human large cell lung carcinoma line decreased tumor metastasis in nude mice PMID: 11807786
- PTX3 directly interacts with inter-alpha-trypsin inhibitor and has a role in hyaluronan organization and cumulus oophorus expansion PMID: 17675295
- The first systematic analysis on the differential expression of ITIH genes in human cancer, showing frequent downregulation that may be associated with initiation and/or progression of these malignancies. PMID: 18226209
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亞細胞定位：Secreted.
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蛋白家族：ITIH family
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數據庫鏈接：
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