Human hepatocyte growth factor activator(HGFA) ELISA kit

Human hepatocyte growth factor activator(HGFA) ELISA kit

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中文名稱：

人肝細胞生長因子激活物(HGFA)酶聯免疫試劑盒
貨號：

CSB-E13160h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人肝細胞生長因子激活物(HGFA)酶聯免疫試劑盒（CSB-E13160h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HGFAC含量。HGFAC即肝細胞生長因子激活物前體，是一種絲氨酸蛋白酶前體，在肝臟合成后釋放入血。其可被凝血酶等激活，進而激活肝細胞生長因子，在細胞生長、運動和形態發生等生理過程及腫瘤侵襲轉移等病理過程有研究，是潛在治療靶點。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，本試劑盒適用于基礎醫學研究領域，例如在肝病模型、腫瘤微環境或創傷修復研究中動態監測HGFA的表達變化，也可用于評估藥物干預對HGFA信號通路的影響，為相關疾病的分子機制探索及治療策略開發提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

HGFAC ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
縮寫：

HGFAC
Uniprot No.：

Q04756
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	95
	Range %	91-99
1:4	Average %	87
	Range %	82-93
1:8	Average %	105
	Range %	101-109

回收率：

The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-91
EDTA plasma (n=4)	94	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.117	2.148	2.133	2.025
2500	1.696	1.682	1.689	1.581
1250	1.215	1.268	1.242	1.134
625	0.834	0.816	0.825	0.717
312	0.502	0.536	0.519	0.411
156	0.265	0.258	0.262	0.154
78	0.168	0.171	0.170	0.062
0	0.106	0.109	0.108

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Plasma macrophage-stimulating protein and hepatocyte growth factor levels are associated with prostate cancer progression Sugie S. et al,Hum Cell,2015

功能：

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
基因功能參考文獻：
1. the HGF-activator has a role in multiple myeloma cells' catalysis of hepatocyte growth factor (HGF) activation PMID: 15172968
2. HGF/MET signaling and aberrant HGF-activator expression is associated with diffuse large B-cell lymphoma PMID: 16189274
3. KLK4 and KLK5 activate pro-HGFA. PMID: 18221492
4. Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
5. HAI-1 (serine peptidase inhibitor, Kunitz type 1) specifically traps active form of HGF activator on cellular surface and regulates HGF activation in pericellular microenvironment. PMID: 11013244
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亞細胞定位：

Secreted. Note=Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
蛋白家族：

Peptidase S1 family
組織特異性：

Liver.
數據庫鏈接：

HGNC: 4894

OMIM: 604552

KEGG: hsa:3083

STRING: 9606.ENSP00000372224

UniGene: Hs.104

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.