Human hepatocyte growth factor activator(HGFA) ELISA kit
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中文名稱：人肝細(xì)胞生長(zhǎng)因子激活物(HGFA)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E13160h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人肝細(xì)胞生長(zhǎng)因子激活物(HGFA)酶聯(lián)免疫試劑盒（CSB-E13160h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HGFAC含量。HGFAC即肝細(xì)胞生長(zhǎng)因子激活物前體，是一種絲氨酸蛋白酶前體，在肝臟合成后釋放入血。其可被凝血酶等激活，進(jìn)而激活肝細(xì)胞生長(zhǎng)因子，在細(xì)胞生長(zhǎng)、運(yùn)動(dòng)和形態(tài)發(fā)生等生理過程及腫瘤侵襲轉(zhuǎn)移等病理過程有研究，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，本試劑盒適用于基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域，例如在肝病模型、腫瘤微環(huán)境或創(chuàng)傷修復(fù)研究中動(dòng)態(tài)監(jiān)測(cè)HGFA的表達(dá)變化，也可用于評(píng)估藥物干預(yù)對(duì)HGFA信號(hào)通路的影響，為相關(guān)疾病的分子機(jī)制探索及治療策略開發(fā)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：HGFAC ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
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縮寫：HGFAC
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：78 pg/mL-5000 pg/mL
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靈敏度：19.5 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cardiovascular
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 81-89 1:2 Average % 95 Range % 91-99 1:4 Average % 87 Range % 82-93 1:8 Average % 105 Range % 101-109 -
回收率：
The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-91 EDTA plasma (n=4) 94 91-99 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 5000 2.117 2.148 2.133 2.025 2500 1.696 1.682 1.689 1.581 1250 1.215 1.268 1.242 1.134 625 0.834 0.816 0.825 0.717 312 0.502 0.536 0.519 0.411 156 0.265 0.258 0.262 0.154 78 0.168 0.171 0.170 0.062 0 0.106 0.109 0.108 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
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靶點(diǎn)詳情
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功能：Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
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基因功能參考文獻(xiàn)：
- the HGF-activator has a role in multiple myeloma cells' catalysis of hepatocyte growth factor (HGF) activation PMID: 15172968
- HGF/MET signaling and aberrant HGF-activator expression is associated with diffuse large B-cell lymphoma PMID: 16189274
- KLK4 and KLK5 activate pro-HGFA. PMID: 18221492
- Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
- HAI-1 (serine peptidase inhibitor, Kunitz type 1) specifically traps active form of HGF activator on cellular surface and regulates HGF activation in pericellular microenvironment. PMID: 11013244
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亞細(xì)胞定位：Secreted. Note=Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
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蛋白家族：Peptidase S1 family
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組織特異性：Liver.
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數(shù)據(jù)庫(kù)鏈接：
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