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Human cytokeratin 18,CK-18 ELISA Kit

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人細胞角蛋白18(CK-18)酶聯免疫試劑盒（CSB-E09172h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物、cell culturesupernates樣本中的KRT18含量。KRT18，全稱角蛋白18，是一種在非鱗狀上皮細胞中表達的I型中間絲蛋白，參與細胞結構和細胞凋亡等過程。其研究機制涉及細胞凋亡、炎癥、癌癥和纖維化等信號通路，與多種疾病如肝硬化、椎間盤退變等密切相關。試劑盒檢測范圍為0.625 ng/mL-15 ng/mL，體外藥物毒性評估、腫瘤微環境研究、組織損傷模型的科研、上皮相關疾病的分子機制探索、生物標志物篩選本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

Cell proliferation inducing gene 46 protein ELISA Kit; Cell proliferation inducing protein 46 ELISA Kit; Cell proliferation-inducing gene 46 protein ELISA Kit; CK 18 ELISA Kit; CK-18 ELISA Kit; CK18 ELISA Kit; CYK 18 ELISA Kit; CYK18 ELISA Kit; Cytokeratin 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Cytokeratin-18 ELISA Kit; K 18 ELISA Kit; K18 ELISA Kit; K1C18_HUMAN ELISA Kit; KA18 ELISA Kit; Keratin 18 ELISA Kit; Keratin 18, type I ELISA Kit; Keratin D ELISA Kit; keratin, type I cytoskeletal 18 ELISA Kit; Keratin-18 ELISA Kit; Krt18 ELISA Kit

縮寫：

KRT18

Uniprot No.：

P05783

種屬：

Homo sapiens (Human)

樣本類型：

serum, plasma, tissue homogenates, cell lysates, cell culture supernates

檢測范圍：

0.625 ng/mL-15 ng/mL

靈敏度：

0.25 ng/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Signal Transduction

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	92
	Range %	88-99
1:4	Average %	97
	Range %	92-106
1:8	Average %	94
	Range %	86-103

回收率：

The recovery of human CK-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
15	1.932	1.958	1.945	1.898
7.5	1.318	1.357	1.338	1.291
3.75	0.786	0.798	0.792	0.745
1.25	0.375	0.362	0.369	0.322
0.625	0.216	0.223	0.220	0.173
0	0.046	0.047	0.047

本試劑盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-glucagon antibody.
Six vials standard (5ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated glucagon antibody (6 ml/bottle) --- Bind to the glucagon in the samples or standards and react with the substrate to make the solution chromogenic.
One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
One vial Stop Solution (7ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.