Human activated protein C,APC ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人活化蛋白C(APC)酶聯(lián)免疫試劑盒（CSB-E09909h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APC含量。APC（腺瘤性結(jié)腸息肉病基因）是一種腫瘤抑制蛋白，作為Wnt信號傳導(dǎo)途徑的拮抗劑，其突變與家族性腺瘤性息肉病（FAP）和結(jié)直腸癌的發(fā)生密切相關(guān)。研究APC機(jī)制主要關(guān)注其如何通過調(diào)節(jié)Wnt通路、細(xì)胞遷移、粘附、轉(zhuǎn)錄激活和細(xì)胞凋亡等過程，影響腫瘤的發(fā)生和發(fā)展。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于凝血調(diào)控機(jī)制、炎癥相關(guān)疾病模型或藥物干預(yù)等基礎(chǔ)研究；在血栓性疾病、敗血癥及腫瘤微環(huán)境等研究領(lǐng)域具有重要價值本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

APC
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-96
1:200	Average %	94
	Range %	90-97
1:400	Average %	97
	Range %	94-103
1:800	Average %	94
	Range %	90-98

回收率：

The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	87	84-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.