Human Vitamin D Receptor,VD R ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人維生素D受體(VD R)酶聯(lián)免疫試劑盒（CSB-E05136h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的VDR含量。VDR即維生素D受體，在人體多個組織器官廣泛分布。它能與活性維生素D結(jié)合形成復(fù)合物，調(diào)控相關(guān)基因表達(dá)。其研究機(jī)制主要圍繞調(diào)節(jié)鈣磷代謝、細(xì)胞增殖分化及免疫功能等方面，對骨骼健康、腫瘤防治等領(lǐng)域研究意義重大。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于生命科學(xué)領(lǐng)域研究，可用于探索維生素D代謝調(diào)控機(jī)制、骨代謝疾病模型構(gòu)建、免疫相關(guān)信號通路分析等實驗場景，支持體外樣本中VDR蛋白的定量檢測需求，為科研人員提供高效可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
1 25 dihydroxyvitamin D3 receptor ELISA Kit; 1 ELISA Kit; 1,25 dihydroxyvitamin D3 receptor ELISA Kit; 1,25-@dihydroxyvitamin D3 receptor ELISA Kit; 25-dihydroxyvitamin D3 receptor ELISA Kit; Member 1 ELISA Kit; NR1I1 ELISA Kit; Nuclear receptor subfamily 1 group I member 1 ELISA Kit; PPP1R163 ELISA Kit; Protein phosphatase 1, regulatory subunit 163 ELISA Kit; VDR ELISA Kit; VDR_HUMAN ELISA Kit; Vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit; Vitamin D hormone receptor ELISA Kit; Vitamin D nuclear receptor variant 1 ELISA Kit; Vitamin D receptor ELISA Kit; Vitamin D3 receptor ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates, cell lysates
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檢測范圍：
6.25 pg/mL-400 pg/mL
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靈敏度：
1.56 pg/mL
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Cancer
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 87-96 | |
| 1:2 | Average % | 103 | |
| Range % | 97-105 | |
| 1:4 | Average % | 95 | |
| Range % | 90-100 | |
| 1:8 | Average % | 94 | |
| Range % | 88-98 | |
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回收率：
| The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 82-94 | |
| EDTA plasma (n=4) | 99 | 90-105 | |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.178 | 2.098 | 2.138 | 1.977 | | | 200 | 1.562 | 1.644 | 1.603 | 1.442 | | | 100 | 1.138 | 1.118 | 1.128 | 0.967 | | | 50 | 0.811 | 0.803 | 0.807 | 0.646 | | | 25 | 0.452 | 0.460 | 0.456 | 0.295 | | | 12.5 | 0.325 | 0.315 | 0.320 | 0.159 | | | 6.25 | 0.227 | 0.231 | 0.229 | 0.068 | | | 0 | 0.162 | 0.160 | 0.161 | | |
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本試劑盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Asociación de los polimorfismos en VDR con la expresión de su mRNA, niveles séricos de calcidiol, calcitriol, sVDR y citocinas Th17/Treg en pacientes con artritis reumatoide
MENCMR ESCOTO,UNIVERSIDAD DE GUADALAJARA,/
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Analysis of Potential Vitamin D Molecule Biomarkers: Association of Calcitriol and Its Hydroxylation Efficiency Ratio with Cardiovascular Disease Risk in Rheumatoid Arthritis Patients
I Demir,Biomedicines,2024
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The Influence of Single Nucleotide Polymorphisms on Vitamin D Receptor Protein Levels and Function in Chronic Liver Disease
E Tourkochristou,International Journal of Molecular Sciences,2023
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Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study
AD Meca,Experimental and Therapeutic Medicine,2021
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Maternal vitamin D deficiency impact on LCPUFA and pregnancy outcome associated with alterations in the one carbon metabolism
AA Nandi,Nutrition Research,2020
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Associations between Vitamin D and Type 2 Diabetes Mellitus: The Role of Vitamin D Receptor and Binding Protein
ES Arafat,Journal of Diabetes Mellitus,2020
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Effects of 1,25-Dihydroxy vitamin D3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors
Avcioglu G, et al,Molecular and Cellular Biochemistry,2019
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Reduced 25-OH vitamin D in patients with autoimmune cytopenias, clinical correlations and literature review
Bruno Fattizzo.et al,Autoimmunity Reviews,2016
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Immunomodulatory effects of vitamin D in peripheral blood monocyte-derived macrophages from patients with rheumatoid arthritis
Neve A et al,Clin Exp Med,2013
靶點詳情
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功能：
Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis.
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基因功能參考文獻(xiàn)：
- Vitamin D Receptor Gene SNPs and the environment interact to Influence survival in hemodialysis patients. PMID: 30087217
- Results suggest the association between some maternal VDR polymorphisms with neonatal anthropometric measures and the risk of premature birth. PMID: 30150529
- SNPs of the VDR and GC genes are associated with vitamin D deficiency in postmenopausal Mexican women. PMID: 30150596
- VDR gene FokI polymorphism is associated with papillary thyroid cancer. PMID: 30486759
- No significant associations were found between the VDR polymorphisms analysed and Developmental dysplasia of the hip . Further work need to be performed using genomewide analysis to elucidate the genetic basis of Developmental dysplasia of the hip . PMID: 30262704
- There was no significant association detected between BMI and rs1544410 of VDR in the Emirati population PMID: 29343214
- Apparently, VDR-mediated signaling pathways seem to be dysregulated in those pathological conditions PMID: 30096760
- Vitamin D Receptorgene TaqI and BsmI polymorphisms might contribute to the increased risk of hallux valgus in Chinese population. Apal or Fokl polymorphisms showed no increased susceptibility. PMID: 29705233
- PTPN2, an anti-inflammatory factor regulated by VDR, was reduced in type 2 diabetics with chronic kidney disease stages 1-2. PMID: 30246029
- ApaI gene polymorphism and Fok1 FF genotype were associated with renal cell carcinoma susceptibility in Asians PMID: 29970659
- findings show polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D. PMID: 29966312
- only VDR FokI polymorphism is associated with Hashimoto's thyroiditis risk in Asian population, but not in Caucasians; and the TaqI, ApaI and BsmI polymorphisms have not positive association neither in the overall population (Meta-Analysis) PMID: 28134349
- Loss of function VDR mutation is associated with Hereditary 1,25-dihydroxyvitamin D-resistant rickets. PMID: 29949513
- JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma. PMID: 29423673
- Associations between VDR gene polymorphisms and osteoporosis risk and bone mineral density in postmenopausal women have been documented. (Meta-analysis) PMID: 29343720
- Vitamin D deficiency and vitamin D receptor variants in mothers and their neonates are risk factors for neonatal sepsis PMID: 29530503
- Study identified that CCC and TCC VDR haplotypes are risk factors for diabetic nephropathy in patients with diabetes type 2. PMID: 30315926
- The VDR rs2228570 variant may increase susceptibility to dyslipidemia in the Chinese Han population. PMID: 30119682
- NB-UVB phototherapy is associated with improved cutaneous VDR expression and vitamin D synthesis. Better repigmentation response to NB-UVB may be related to higher baseline VDR expression and its upregulation after phototherapy PMID: 29080365
- Vitamin D Receptor Gene Polymorphism is associated with Breast Cancer. PMID: 28780723
- Studied association of vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs) and promoter region deletions of toll like receptor 2 (TLR2) with genetic predisposition for pulmonary tuberculosis (PTB) in India communities. Results show that the BsmI and FokI polymorphisms of the VDR gene are significantly associated with an increased risk of PTB. PMID: 29727015
- Results disclose FokI polymorphism as a relevant variant capturing the association of VDR polymorphisms with viral infection. PMID: 30092343
- VDR (rs1544410) SNP was found to be associated with decreased serum (25[OH]D) levels. PMID: 29738868
- CA genotype of ApaI VDR gene polymorphism was associated with family history and C allele of ApaI was related with family history and hypercalciuria in under one-year-old infants from Turkey. PMID: 29085969
- A total of six Bcell epitopes and three Tcell epitopes for VDR were predicted by bioinformatics, which when validated, may in the future aid in immunological diagnosis and development of a targeted drug therapy for clinical asthma. PMID: 29901144
- Review/Meta-analysis: VDR Tru9I polymorphism may be associated with osteoporosis risk in Chinese individuals, but BsmI, ApaI polymorphisms might not be a risk factor for osteoporosis. PMID: 29624920
- Our data reveal that VDR plays a central role in protecting cells from excessive respiration and production of ROS that leads to cell damage. PMID: 29874855
- This study emphasizes a positive association between SNPs (Fok-I and Bsm-I) and T1DM among Saudi children with increased risk with the Fok-I F and Bsm-I b alleles. PMID: 29417618
- The Apa-I variant in VDR gene is associated with metabolic syndrome in southern Brazilian women with polycystic ovary syndrome. PMID: 29669566
- Whole blood VDR gene expression was significantly higher in the autistic disorder group compared to control subjects (p < 0.0001). There were no significant differences among allele and genotype distribution of rs11568820 and rs4516035 polymorphisms between autistic disorder patients and controls. PMID: 29777458
- Vitamin D receptor ApaI AC genotype may be a possible cardiovascular risk factor for the development of arteriovenous fistula failure. PMID: 29544394
- preliminary results indicate the VDR gene ApaI, BsmI, FokI, and TaqI polymorphisms may not be associated with elevated multiple sclerosis (MS) risk among overall populations, but ApaI polymorphism may confer different susceptibility to MS among different populations - systematic review and meta-analysis PMID: 29110148
- Studied association between 25-hydroxy vitamin D (25[OH]D) levels and vitamin D receptor (VDR) gene polymorphism in association with diabetes type 2. PMID: 28739347
- The VDR Tru9I 'uu' genotype may increase the risk of premenopausal breast cancer. PMID: 29529900
- Low VDR expression is associated with Coronary Artery Disease. PMID: 29176261
- Expression analyses showed significant downregulation of VDR expression in peripheral blood of epileptic patients compared with healthy subjects. PMID: 29549592
- This meta-analysis demonstrated the association between FokI and ApaI polymorphisms in VDR gene with the risk of BD, providing insights into the potential role of vitamin D receptor in the pathogenesis of BD. PMID: 29388852
- Vitamin D receptor polymorphisms is a risk factor for multiple sclerosis susceptibility and progression in the Czech population. PMID: 29589202
- important role for SOST SNP rs1877632 and VDR SNPs rs10735810 and rs731236 in the pathophysiology of stress fracture PMID: 29129460
- CT genotype and the C allele of VDR were significantly associated with increased risks of childhood autism spectrum disorder. PMID: 29581796
- Study found a significant association between multiple sclerosis and the VDR FokI polymorphism in our region of Turkey. PMID: 29331875
- VDR's Fok-I and Taq-I show significant association with risk of RRMS, while Apa-I and Bsm-I are not related to the risk of the disease in Iranian Kurds. PMID: 29072967
- The VDR rs2228570 polymorphism increases the risk of ovarian cancer in Caucasian populations in a dominant genetic model. PMID: 29239065
- The present study indicates an association between VDR and vitamin D binding protein Single Nucleotide Polymorphisms and Type 1 Diabetes Mellitus among Turkish subjects. PMID: 29506625
- Review/Meta-analysis: VDR B allele, and BB + Bb genotypes of Bsm I variant, Tt genotype of Taq I variant might be risk factors for diabetic nephropathy. PMID: 28703918
- The VDR Bb genotype is an independent predictor of developing secondary hyperparathyroidism in patients with end stage kidney disease. PMID: 29415666
- FokI and TaqI VDR variants are significantly associated with systemic lupus erythematosus in an eastern Indian cohort. PMID: 29230954
- examination of the evidence for the role of Vitamin D Receptor (VDR) Polymorphisms in autoimmune diseases (review). PMID: 28786260
- results suggests that there may be a relationship between certain VDR genotype combinations and the risk of preterm birth. PMID: 27958635
- VDR BsmI polymorphism was associated with decreased risk of periodontitis in Chinese individuals from South China (meta-analysis). PMID: 29208185
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相關(guān)疾病：
Rickets vitamin D-dependent 2A (VDDR2A)
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亞細(xì)胞定位：
Nucleus. Cytoplasm.
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蛋白家族：
Nuclear hormone receptor family, NR1 subfamily
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數(shù)據(jù)庫鏈接：
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