Human V-set and immunoglobulin domain-containing protein 4(VSIG4) ELISA kit

Human V-set and immunoglobulin domain-containing protein 4(VSIG4) ELISA kit

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中文名稱：

人含V-set和Ig結(jié)構(gòu)域蛋白4(VSIG4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL025928HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人含V-set和Ig結(jié)構(gòu)域蛋白4(VSIG4)酶聯(lián)免疫試劑盒（CSB-EL025928HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的VSIG4含量。VSIG4作為靶點(diǎn)，在免疫調(diào)節(jié)等方面具重要背景意義。其研究機(jī)制主要圍繞免疫相關(guān)通路，它可與相關(guān)配體結(jié)合，調(diào)節(jié)免疫細(xì)胞功能，影響免疫反應(yīng)進(jìn)程，在腫瘤免疫、炎癥疾病等研究領(lǐng)域受關(guān)注，有望為疾病治療提供新靶點(diǎn)與策略。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研場(chǎng)景中探究VSIG4在免疫微環(huán)境動(dòng)態(tài)變化、疾病模型構(gòu)建及藥物干預(yù)效果評(píng)估中的作用。例如，在腫瘤免疫研究中可分析VSIG4與免疫檢查點(diǎn)分子間的協(xié)同調(diào)控機(jī)制，在自身免疫性疾病模型中可監(jiān)測(cè)巨噬細(xì)胞功能狀態(tài)，或在炎癥相關(guān)實(shí)驗(yàn)中評(píng)估組織特異性免疫抑制信號(hào)的變化趨勢(shì)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Complement receptor of the immunoglobulin superfamily ELISA Kit; CRIg ELISA Kit; Ig superfamily protein ELISA Kit; Protein Z39Ig ELISA Kit; UNQ317/PRO362 ELISA Kit; V set and immunoglobulin domain containing 4 ELISA Kit; V-set and immunoglobulin domain-containing protein 4 ELISA Kit; VSIG4 ELISA Kit; VSIG4_HUMAN ELISA Kit; Z39IG ELISA Kit
縮寫：

VSIG4
Uniprot No.：

Q9Y279
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VSIG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-94
1:2	Average %	98
	Range %	91-104
1:4	Average %	97
	Range %	94-105
1:8	Average %	96
	Range %	86-100

回收率：

The recovery of human VSIG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-97
EDTA plasma (n=4)	90	86-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.298	2.154	2.226	2.035
1000	1.557	1.501	1.529	1.338
500	1.025	1.080	1.053	0.862
250	0.700	0.675	0.688	0.497
125	0.510	0.490	0.500	0.309
62.5	0.355	0.349	0.352	0.161
31.25	0.236	0.233	0.235	0.044
0	0.192	0.189	0.191

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Proteomic profiling identifies SPP1 associated with rapidly progressive interstitial lung disease in anti-MDA5-positive dermatomyositis T Huynh,Arthritis research & therapy,2024
Immunoregulatory protein V‐set and immunoglobulin domain‐containing 4 is overexpressed in patients with endometriosis GH Jeon,J Obstet Gynaecol Res,2020

功能：

Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
基因功能參考文獻(xiàn)：
1. Soluble VSIG4 levels are associated with the progression and recurrence of ovarian cancer, indicating that soluble VSIG4 may be used as a potential biomarker for predicting tumor prognosis. PMID: 28498255
2. VSIG4 signaling provides an anti-immune evasion mechanism that prevents the outgrowth of intracellular bacteria in macrophages PMID: 27440002
3. The VSIG4 upregulation by LMP1 was regulated at the transcriptional level via the NF-kB signaling axis. PMID: 28859984
4. VSIG4 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
5. we concluded that let-7g-5p inhibits epithelial-mesenchymal transition (EMT) consistent with reduction of glioma stem cell (GSC) phenotypes by targeting VSIG4 in glioblastoma. PMID: 27634309
6. Data indicate that rotein kinase calpha (PKCalpha) plays a role in downregulating complement receptor Ig (CRIg coded by V-set and Ig domain-containing protein 4 VSIG4) expression. PMID: 25687755
7. complement receptor of the immunoglobulin superfamily-L-factor H protects glomerular mesangial cells from complement-mediated injury and proliferative lesions PMID: 25114177
8. we identified VSIG4 as a potential diagnostic marker of severe preeclampsia. The determination of this gene may improve the prognostic assessment of severe preeclampsia. PMID: 24349325
9. Data indicate that massive V-set and Ig domain-containing 4 VSIG4(+) cell infiltration throughout the non-small-cell lung cancer samples. PMID: 24862966
10. we showed that a complement receptor of the Ig superfamily (CRIg, also known as Z39Ig), a receptor for complement fragments (C3b and iC3b), was expressed on a subset of intestinal macrophages in murine and human large intestine PMID: 21768202
11. These results suggest that T cells can opposite T cell hyporesponsiveness through dampening Z39Ig inhibitory signals from macrophages and thus maintain their anti-viral function in chronic hepatitis B. PMID: 20399148
12. hVSIG4 recombinant adenovirus-transfected DCs suppress T cell proliferation, cytokine production and activation marker expression with PMID: 19914289
13. Results report the identification and characterization of a Complement Receptor of the Immunoglobulin superfamily, CRIg, that binds complement fragments C3b and iC3b. PMID: 16530040
14. These data indicate that the macrophage Z39Ig is involved in the pathogenesis of inflammatory diseases through chemokine induction, which will promote the migration of inflammatory cells into the lesion area, and MMP-9 induction. PMID: 16882875
15. The specific expression of VSIG4 on resting macrophages suggests that VSIG4 may be important for the maintenance of T cell unresponsiveness in healthy tissues. PMID: 17016562
16. CRIg is not only a phagocytic receptor, but also a potent inhibitor of the alternative pathway convertases PMID: 17051150
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亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
組織特異性：

Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 17032

OMIM: 300353

KEGG: hsa:11326

STRING: 9606.ENSP00000363869

UniGene: Hs.8904

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.