Human Transmembrane protein 95(TMEM95) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人跨膜蛋白95(TMEM95)酶聯免疫試劑盒（CSB-EL023899HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TMEM95含量。人跨膜蛋白95（TMEM95）是一種精子膜蛋白，其敲除在小鼠中會導致男性特異性不育表型。研究發現，TMEM95與卵子膜結合，促進精子與卵子的膜融合，其作用機制尚待進一步研究。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本試劑盒通過高特異性配對抗體實現精準檢測，可兼容多種生物樣本類型，適用于基礎科研中探索TMEM95在生殖系統生理功能、疾病模型中的表達變化，或結合其他分子標記物解析相關調控機制。其寬泛的檢測范圍可滿足不同濃度樣本的定量需求，為體外實驗、動物模型或組織樣本的蛋白表達研究提供可靠工具，助力生殖健康、發育生物學等領域的分子機制探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TMEM95 ELISA Kit; UNQ9390/PRO34281 ELISA Kit; Transmembrane protein 95 ELISA Kit
縮寫：

TMEM95
Uniprot No.：

Q3KNT9
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM95 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	100
	Range %	95-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	89-96

回收率：

The recovery of human TMEM95 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Sperm protein required for fusion of sperm with the egg membrane during fertilization.
亞細胞定位：

Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein.
數據庫鏈接：

HGNC: 27898

OMIM: 617814

KEGG: hsa:339168

STRING: 9606.ENSP00000331466

UniGene: Hs.259432