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Human Semaphorin-3G(SEMA3G) ELISA kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

人臂板蛋白-3G(SEMA3G)酶聯免疫試劑盒（CSB-EL020986HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SEMA3G含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎科研中對SEMA3G表達水平的精準分析；為探索SEMA3G在神經系統發育、腫瘤血管生成或免疫調控中的分子機制提供可靠工具，也可用于評估疾病模型或藥物干預下SEMA3G的濃度變化；支持科研人員在體外實驗中對SEMA3G進行高通量定量檢測，適用于細胞生物學、病理機制研究及生物標志物篩選等非臨床研究場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FLJ00014 ELISA Kit; MGC119473 ELISA Kit; Sem2 ELISA Kit; SEM3G_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3G ELISA Kit; Sema3g ELISA Kit; Semaphorin 3G ELISA Kit; Semaphorin sem2 ELISA Kit; Semaphorin-3G ELISA Kit
縮寫：

SEMA3G
Uniprot No.：

Q9NS98
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-102
1:2	Average %	101
	Range %	96-105
1:4	Average %	94
	Range %	87-98
1:8	Average %	99
	Range %	91-103

回收率：

The recovery of human SEMA3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.