Human Protein FAM132B(FAM132B) ELISA kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人FAM132B蛋白(FAM132B)酶聯免疫試劑盒（CSB-EL008059HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的FAM132B含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外培養細胞模型的功能研究、動物實驗樣本的分子機制探索，以及臨床前研究中生物標志物的定量分析等科研場景，為代謝調控通路、細胞分化機制等基礎研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; ERFE_HUMAN ELISA Kit; Erythroferrone ELISA Kit; FAM132B ELISA Kit; Myonectin ELISA Kit
縮寫：

FAM132B
Uniprot No.：

Q4G0M1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-95
1:2	Average %	95
	Range %	90-101
1:4	Average %	87
	Range %	83-94
1:8	Average %	99
	Range %	94-105

回收率：

The recovery of human FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-102
EDTA plasma (n=4)	88	84-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
基因功能參考文獻：
1. From a clinical point of view, erythroferrone could become a useful biological marker of iron metabolism and a therapeutic target. [review] PMID: 28666715
2. FAM132b expression is upregulated in Congenital Dyserythropoietic Anemia type II. PMID: 27540014
3. In hemodialysis patients DA and CERA increased levels of ERFE that regulated hepcidin 25 and led to iron mobilization from body stores during erythropoiesis. PMID: 26978524
4. Myonectin links skeletal muscle to lipid homeostasis in liver and adipose tissue in response to alterations in energy state, revealing a novel myonectin-mediated metabolic circuit. PMID: 22351773
亞細胞定位：

Secreted.
蛋白家族：

Adipolin/erythroferrone family
數據庫鏈接：

HGNC: 26727

OMIM: 615099

KEGG: hsa:151176

UniGene: Hs.24951