Human Platelet receptor Gi24(C10orf54) ELISA kit

文獻(xiàn)引用 (1) 說明書下載

中文名稱：

人血小板受體Gi24(C10orf54)酶聯(lián)免疫試劑盒
貨號：

CSB-EL002961HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人血小板受體Gi24(C10orf54)酶聯(lián)免疫試劑盒（CSB-EL002961HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的C10orf54含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于心血管疾病機(jī)制研究、血小板功能體外實(shí)驗(yàn)、藥物篩選模型建立等科研場景，為探索Gi24在細(xì)胞信號傳導(dǎo)、病理生理學(xué)調(diào)控中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

B7 H5 ELISA Kit; B7H5 ELISA Kit; C10orf54 ELISA Kit; chromosome 10 open reading frame 54 ELISA Kit; DD1alpha ELISA Kit; GI24 ELISA Kit; GI24_HUMAN ELISA Kit; PD-1H ELISA Kit; PDCD1 homolog ELISA Kit; Platelet receptor Gi24 ELISA Kit; PP2135 ELISA Kit; sisp 1 ELISA Kit; SISP1 ELISA Kit; stress induced secreted protein 1 ELISA Kit; UNQ730/PRO1412 ELISA Kit; V domain Ig suppressor of T cell activation ELISA Kit; V set domain containing immunoregulatory receptor ELISA Kit; V set immunoregulatory receptor ELISA Kit; V type immunoglobulin domain containing suppressor of T cell activation ELISA Kit; VISTA ELISA Kit
縮寫：

C10orf54
Uniprot No.：

Q9H7M9
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C10orf54 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	83-89
1:2	Average %	100
	Range %	95-105
1:4	Average %	84
	Range %	81-89
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human C10orf54 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	99	95-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.366	2.305	2.336	2.213
5	1.642	1.658	1.650	1.527
2.5	0.898	0.868	0.883	0.760
1.25	0.475	0.455	0.465	0.342
0.625	0.320	0.326	0.323	0.200
0.312	0.249	0.251	0.250	0.127
0.156	0.195	0.193	0.194	0.071
0	0.125	0.120	0.123

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Soluble B7-H5 Is a Novel Diagnostic, Severity, and Prognosis Marker in Acute Pancreatitis R Xu,BioMed Research International,2021

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功能：

Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.
基因功能參考文獻(xiàn)：
1. Data indicate that VISTA is predominantly expressed and up-regulated in the high-density infiltrated immune cells but minimal in pancreatic cancerous cells. PMID: 29771768
2. High VISTA expression is associated with colorectal carcinoma. PMID: 30128738
3. VISTA expression supports immune-complex inflammation in collagen antibody-induced arthritis and VISTA is expressed in human synovium PMID: 29216931
4. This study is the first to describe the expression of VISTA-expressing lymphocytes in melanoma samples and in the context of acquired resistance to immune checkpoint inhibitors PMID: 28776578
5. Taken together, the results indicated that the VISTA high and CD8 low group, as an immunosuppressive subgroup, might be associated with a poor prognosis in primary OSCC. These findings indicated that VISTA might be a potential immunotherapeutic target in OSCC treatment. PMID: 28236118
6. this review describes the functions of VISTA in the context of cancer immunotherapy PMID: 28258694
7. overexpression of the newly described co-stimulatory molecule, PD1 homologue (PD-1H) in human monocyte/macrophages is sufficient to induce spontaneous secretion of multiple cytokines. PMID: 25279955
8. p53-induced expression of DD1alpha prevents persistence of cell corpses and ensures efficient generation of precise immune responses in mice. PMID: 26228159
9. Results suggest that GI24 contributes to tumor-invasive growth in the collagen matrix by augmenting cell surface MT1-MMP. PMID: 20666777
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亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein.
組織特異性：

Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta
數(shù)據(jù)庫鏈接：

HGNC: 30085

OMIM: 615608

KEGG: hsa:64115

STRING: 9606.ENSP00000378409

UniGene: Hs.47382

Human Platelet receptor Gi24(C10orf54) ELISA kit

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