Human Phospholipase A1 member A(PLA1A) ELISA kit

說明書下載

中文名稱：

人磷脂酶A1成員A(PLA1A) 酶聯免疫試劑盒
貨號：

CSB-EL018084HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人磷脂酶A1成員A(PLA1A) 酶聯免疫試劑盒（CSB-EL018084HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PLA1A含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品適用于基礎科研領域，如探究PLA1A在脂代謝相關疾病模型中的動態變化、評估其在炎癥反應中的作用機制，或用于細胞及動物實驗中蛋白表達水平的定量比較分析，為脂質代謝與相關疾病的分子機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AA986889 ELISA Kit; NMD ELISA Kit; OTTHUMP00000215248 ELISA Kit; OTTHUMP00000215249 ELISA Kit; OTTHUMP00000215250 ELISA Kit; Phosphatidylserine specific phospholipase A1 ELISA Kit; Phosphatidylserine specific phospholipase A1alpha ELISA Kit; Phosphatidylserine-specific phospholipase A1 ELISA Kit; Phospholipase A1 member A ELISA Kit; pla1a ELISA Kit; PLA1A_HUMAN ELISA Kit; PS PLA1 ELISA Kit; PS-PLA1 ELISA Kit; PSPLA 1 ELISA Kit; PSPLA1 ELISA Kit
縮寫：

PLA1A
Uniprot No.：

Q53H76
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	97
	Range %	93-101
1:4	Average %	99
	Range %	95-106
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of human PLA1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	99	95-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.340	2.344	2.342	2.237
10	1.604	1.741	1.673	1.568
5	0.936	0.968	0.952	0.847
2.5	0.576	0.554	0.565	0.460
1.25	0.353	0.325	0.339	0.234
0.625	0.230	0.213	0.222	0.117
0.312	0.185	0.180	0.183	0.078
0	0.104	0.106	0.105

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Hydrolyzes the ester bond of the acyl group attached at the sn-1 position of phosphatidylserines (phospholipase A1 activity) and 1-acyl-2-lysophosphatidylserines (lysophospholipase activity) in the pathway of phosphatidylserines acyl chain remodeling. Cleaves phosphatidylserines exposed on the outer leaflet of the plasma membrane of apoptotic cells producing 2-acyl-1-lysophosphatidylserines, which in turn enhance mast cell activation and histamine production. Has no activity toward other glycerophospholipids including phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids or phosphatidylinositols, or glycerolipids such as triolein.; Hydrolyzes lyso-PS but not PS.
基因功能參考文獻：
1. Serum PS-PLA1 levels were significantly higher in the melanoma subjects and associated with the clinical stages. PMID: 29500864
2. the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA1-mediated pathway might be involved in the production of LysoPS in gastric cancer. PMID: 28143894
3. PS-PLA1 expression in colorectal cancer is associated with tumor invasion and metastasis. PMID: 25750298
4. These data suggest that PLA1A plays an important role in bridging the membrane-associated NS2-E2 complex and the NS5A-associated replication complex via its interaction with hepatitis C virus E2, NS2, and NS5A. PMID: 25505071
5. PLA1A2 polymorphism is associated with mortality in participants whose hemoglobin A1c ranges from 5.5% to 6.5%. PMID: 24886443
6. Overall, our study shed the light on new structural features of the phospholipase activity of pancreatic lipase family members. PMID: 24368210
7. These results suggest that the expression of PS-PLA(1) mRNA in THP-1-derived macrophages is activated via TLR4. PMID: 20573295
8. polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 PMID: 12436198
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亞細胞定位：

Secreted.
蛋白家族：

AB hydrolase superfamily, Lipase family
組織特異性：

Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.
數據庫鏈接：

HGNC: 17661

OMIM: 607460

KEGG: hsa:51365

STRING: 9606.ENSP00000273371

UniGene: Hs.437451

Human Phospholipase A1 member A(PLA1A) ELISA kit

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