Human Myostatin,MSTN ELISA Kit
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中文名稱：
人肌抑素(MSTN)酶聯(lián)免疫試劑盒
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貨號：
CSB-E11300h
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規(guī)格：
96T/48T
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價格：
￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：
人肌抑素(MSTN)酶聯(lián)免疫試劑盒（CSB-E11300h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MSTN含量。MSTN（肌肉生長抑制素）是轉(zhuǎn)化生長因子β家族成員，主要調(diào)節(jié)骨骼肌生長和發(fā)育。研究顯示，MSTN缺失可促進骨骼肌生長，并改變腸道微生物群組成，其機制可能與腸道微生物代謝產(chǎn)物戊酸通過GPR43激活A(yù)kt/mTOR信號通路有關(guān)。試劑盒檢測范圍為0.625 ng/mL-20 ng/mL，該產(chǎn)品適用于探究肌肉發(fā)育機制、運動代謝調(diào)控、肌肉萎縮相關(guān)疾病模型等基礎(chǔ)研究領(lǐng)域，尤其可為運動科學(xué)、肌肉再生及代謝性疾病（如肥胖、Ⅱ型糖尿病）的分子機制研究提供可靠檢測工具。通過檢測不同生理或病理條件下生物樣本中MSTN的濃度變化，研究人員可深入解析其在肌肉 - 脂肪代謝網(wǎng)絡(luò)中的動態(tài)調(diào)控作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
Cmpt ELISA Kit; GDF-8 ELISA Kit; GDF8_HUMAN ELISA Kit; Growth/differentiation factor 8 ELISA Kit; MSLHP ELISA Kit; MSTN ELISA Kit; MSTN myostatin ELISA Kit; Myostatin ELISA Kit; Myostatin Propeptide ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
0.625 ng/mL-20 ng/mL
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靈敏度：
0.312 ng/mL
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Signal Transduction
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
92-99 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:8 |
Average % |
86 |
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| Range % |
82-90 |
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回收率：
| The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
87-97 |
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| EDTA plasma (n=4) |
86 |
82-91 |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
1.851 |
1.992 |
1.922 |
1.878 |
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| 10 |
1.058 |
1.136 |
1.097 |
1.053 |
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| 4.375 |
0.502 |
0.524 |
0.513 |
0.469 |
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| 1.875 |
0.268 |
0.255 |
0.262 |
0.218 |
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| 0.625 |
0.116 |
0.119 |
0.118 |
0.074 |
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| 0 |
0.043 |
0.045 |
0.044 |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MSTN antibody.
- Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated MSTN antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
- Substrate A (1 x 7 ml)
- Substrate B (1 x 7 ml)
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻
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Supplementing With Which Form of Creatine (Hydrochloride or Monohydrate) Alongside Resistance Training Can Have More Impacts on Anabolic/Catabolic Hormones, Strength and Body Composition?
E Eghbali, H Arazi, K Suzuki,Physiological Research,2024
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Novel Approach for Glycemic Management Incorporating Vibration Stimulation of Skeletal Muscle in Obesity
M Kim,International Journal of Environmental Research and Public Health,2023
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Effects of functional training with blood occlusion on the irisin, follistatin, and myostatin myokines in elderly men
F Pazokian,European Review of Aging and Physical Activity,2022
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Strategic Ingestion of High-Protein Dairy Milk during a Resistance Training Program Increases Lean Mass, Strength, and Power in Trained Young Males
Maryam Pourabbas,Nutrients,2021
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Comparison of whole egg v. egg white ingestion during 12 weeks of resistance training on skeletal muscle regulatory markers in resistance-trained men
R Bagheri,Br J Nutr,2020
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Urinary Levels of titin-n fragment, a Skeletal Muscle Damage Marker, are increased in Subjects with nonalcoholic fatty Liver Disease
Oshida N,Scientific Reports,2019
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Blood Flow Restriction During Futsal Training Increases Muscle Activation and Strength
Sadegh Amani-Shalamzari,Frontiers in Physiology,2019
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High-Intensity Aerobic Exercise Improves Both Hepatic Fat Content and Stiffness in Sedentary Obese Men with Nonalcoholic Fatty Liver Disease
Sechang Oh,Sci Rep. ,2017
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Serum concentration of myostatin, insulin and glucose response to continuous and discontinuous exercises in overweight menopausal and menstrual women
Masomeh Hannani.et al,Hormozgan Medical Journal,2016
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Myostatin and carbohydrate disturbances
Assyov YS.et al,Endocr Res.,2016
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The correlation of resistance exercise-induced myostatin with insulin resistance and plasma cytokines in healthy young men
Kazemi F,J Endocrinol Invest,2015
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