Human Melanotransferrin(MFI2) ELISA kit

Human Melanotransferrin(MFI2) ELISA kit

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中文名稱：

人黑素轉鐵蛋白(MFI2)酶聯免疫試劑盒
貨號：

CSB-EL013754HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人黑素轉鐵蛋白(MFI2)酶聯免疫試劑盒（CSB-EL013754HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的MFI2含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，特別適用于黑色素瘤細胞機制研究、鐵代謝相關信號通路探索，以及疾病模型構建中生物標志物的定量驗證本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Antigen p97 ELISA Kit; CD228 ELISA Kit; CD228 antigen ELISA Kit; MAP97 ELISA Kit; Melanoma associated antigen p97 ELISA Kit; Melanoma-associated antigen p97 ELISA Kit; Melanotransferrin ELISA Kit; Mfi2 ELISA Kit; MTF1 ELISA Kit; TRFM_HUMAN ELISA Kit
縮寫：

MFI2
Uniprot No.：

P08582
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MFI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	103
	Range %	99-108
1:4	Average %	90
	Range %	85-94
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human MFI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.486	2.315	2.401	2.296
5	2.034	1.974	2.004	1.899
2.5	1.506	1.414	1.460	1.355
1.25	1.027	0.981	1.004	0.899
0.625	0.631	0.663	0.647	0.542
0.312	0.348	0.325	0.337	0.232
0.156	0.241	0.257	0.249	0.144
0	0.107	0.103	0.105

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Level of Melanotransferrin in Tissue and Sera Serves as a Prognostic Marker of Gastric Cancer SAWAKI K, et al,Anticancer Research,2019

功能：

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
基因功能參考文獻：
1. Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID: 25216327
2. PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID: 21190562
3. The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID: 11852110
4. its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID: 12230555
5. membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID: 12750156
6. Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID: 12809550
7. The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID: 15716025
8. melanotransferrin has a role in cell proliferation and tumorigenesis PMID: 16704991
9. Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID: 16713448
10. Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID: 17196552
11. HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID: 17227112
12. Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID: 17449903
13. this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID: 17452986
14. Biochemical and spectroscopic studies of MTF1 are reported. PMID: 18691669
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亞細胞定位：

[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Transferrin family
組織特異性：

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.
數據庫鏈接：

HGNC: 7037

OMIM: 155750

KEGG: hsa:4241

STRING: 9606.ENSP00000296350

UniGene: Hs.184727

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.