Human Macroprolactin ELISA Kit
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中文名稱:人巨泌乳素酶聯免疫試劑盒
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貨號:CSB-E16222h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人巨泌乳素酶聯免疫試劑盒(CSB-E16222h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MPRL含量。人巨泌乳素是一種由垂體前葉分泌的激素,主要功能是刺激乳汁生成和分泌。其靶點背景涉及多種生物過程,包括生殖和母乳喂養。研究機制顯示,人巨泌乳素通過其受體PRLR發揮作用,影響細胞增殖、分化、分泌和生存,并在多種生理和病理生理過程中扮演重要角色。試劑盒檢測范圍為0.4 μIU/mL-20 μIU/mL,可廣泛用于科研領域中對巨泌乳素表達水平的動態監測,例如探究其在細胞分泌調控、組織生理病理狀態中的生物學功能,或用于體外實驗模型中藥物干預、基因編輯等因素對巨泌乳素合成與釋放的影響機制研究。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:MPRL
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:0.4 μIU/mL-20 μIU/mL
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靈敏度:0.5 μIU/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human macroprolactin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 90-98 1:2 Average % 89 Range % 84-99 1:4 Average % 95 Range % 91-102 1:8 Average % 95 Range % 90-103 -
回收率:
The recovery of human macroprolactin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-102 EDTA plasma (n=4) 97 87-108 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μIU/ml OD1 OD2 Average Corrected 20 2.003 2.031 2.017 1.987 8 1.095 1.105 1.100 1.070 3 0.617 0.624 0.621 0.591 1 0.295 0.303 0.299 0.269 0.4 0.145 0.149 0.147 0.117 0 0.029 0.031 0.030 -
數據處理:
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貨期:3-5 working days
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