Human Ly6/PLAUR domain-containing protein 3(LYPD3) ELISA kit

Human Ly6/PLAUR domain-containing protein 3(LYPD3) ELISA kit

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中文名稱：

人Ly6/PLAUR結構域蛋白3(LYPD3)酶聯(lián)免疫試劑盒
貨號：

CSB-EL013263HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人Ly6/PLAUR結構域蛋白3(LYPD3)酶聯(lián)免疫試劑盒（CSB-EL013263HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LYPD3含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本試劑盒適用于腫瘤微環(huán)境研究、細胞間相互作用機制探索或藥物開發(fā)中生物標志物動態(tài)監(jiān)測等科研場景。滿足基礎研究與轉化醫(yī)學中對LYPD3蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

2310061G07Rik ELISA Kit; C4.4A ELISA Kit; GPI-anchored metastasis-associated protein C4.4A homolog ELISA Kit; GPI-anchored metastasis-associated protein homolog ELISA Kit; Ly6/PLAUR domain-containing protein 3 ELISA Kit; Lypd3 ELISA Kit; LYPD3_HUMAN ELISA Kit; Matrigel-induced gene C4 protein ELISA Kit; MIG-C4 ELISA Kit; SHINC3 ELISA Kit
縮寫：

LYPD3
Uniprot No.：

O95274
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LYPD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	94-105
1:2	Average %	88
	Range %	81-93
1:4	Average %	94
	Range %	87-97
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of human LYPD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	85-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.626	2.446	2.536	2.378
1000	2.228	2.113	2.171	2.013
500	1.506	1.488	1.497	1.339
250	0.803	0.785	0.794	0.636
125	0.502	0.491	0.497	0.339
62.5	0.377	0.364	0.371	0.213
31.25	0.246	0.251	0.249	0.091
0	0.156	0.160	0.158

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.
基因功能參考文獻：
1. While in the healthy liver hepatocytes are C4.4A negative, data show that C4.4A is strongly expressed in hepatocellular carcinoma (HCC) with upregulation at the invasive front and in lung metastasis, indicating that C4.4A apparently contributes to HCC progression. PMID: 29048672
2. Our results are consistent with previous data in mouse embryogenesis and wound healing. Based on these findings, we conclude that this human TES model provides an excellent surrogate for studies of C4.4A and Haldisin expressions in human stratified epithelia. PMID: 29075641
3. Expression and crystallographic studies of the D1D2 domains of C4.4A have been reported. PMID: 28777093
4. LYPD3 has a role in the maintenance of colorectal cancer stem-like cells. PMID: 28238780
5. Highly expressed C4.4A protein in HER2-positive human breast cancers indicate a good prognosis. PMID: 23918676
6. overexpression of C4.4A correlates with metastatic potential of gastric cancer and C4.4A could be a novel independent prognostic marker for predicting outcome. PMID: 24935570
7. expression of the Ly6/uPAR-domain proteins C4.4A and Haldisin in non-invasive and invasive skin lesions PMID: 25414274
8. Tenascin-C expression was significantly associated with C4.4A expression in clinical esophageal squamous carcinoma samples suggesting that there may be a functional role for the C4.4A to induceTenascin-C in vivo. PMID: 23708783
9. findings suggest that a tight association between C4.4A and tumor budding may, in part, be due to C4.4A promoting epithelial-mesenchymal transition at the invasive front of colorectal cancer PMID: 22404718
10. the first explanation for the C4.4A contribution to wound healing and metastasis. PMID: 22431918
11. data indicate that expression of the C4.4A protein at the invasive front acts as a novel prognostic marker in colorectal cancer, possibly through invasion-related mechanisms PMID: 20825414
12. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan PMID: 12592373
13. High tumour cell C4.4A expression is associated with shorter survival for non-small cell lung cancer patients. PMID: 17706320
14. Overexpression of C4.4A is associated with invasion and metastasis of esophageal squamous cell carcinoma PMID: 17849475
15. we consider C4.4A as a candidate diagnostic marker in colorectal cancer, which possibly can be detected in body fluids PMID: 17912244
16. cleavage of C4.4A by ADAM10 and ADAM17 contributes to tumor progression PMID: 18979631
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
組織特異性：

Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TC
數(shù)據(jù)庫鏈接：

HGNC: 24880

OMIM: 609484

KEGG: hsa:27076

STRING: 9606.ENSP00000244333

UniGene: Hs.631594

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.