Human L-Carnitine ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人左旋肉堿/L-肉堿酶聯免疫試劑盒（CSB-E13606h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的L-Carnitine含量。L-Carnitine即左旋肉堿，它是一種促使脂肪轉化為能量的類氨基酸，存在于人體中。在研究機制方面，它能將長鏈脂肪酸轉運到線粒體中進行氧化，從而促進脂肪代謝。臨床上還可用于輔助治療一些疾病，在減肥等領域也備受關注。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于代謝調控機制研究、運動醫學評估、營養代謝實驗及藥物開發中的生物標志物檢測；滿足實驗室對脂肪酸代謝、心肌能量代謝及線粒體功能相關研究的精準定量需求；為探索L - 肉堿在能量代謝異常、肥胖癥或運動恢復等領域的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

L-Carnitine
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	88
	Range %	80-96
1:400	Average %	97
	Range %	91-105
1:800	Average %	99
	Range %	92-110
1:1600	Average %	100
	Range %	90-110

回收率：

The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-110
EDTA plasma (n=4)	97	90-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
20	2.749	2.639	2.694	2.543
10	2.010	1.976	1.993	1.842
5	1.303	1.294	1.299	1.148
2.5	0.889	0.839	0.864	0.713
1.25	0.561	0.524	0.543	0.392
0.625	0.371	0.359	0.365	0.214
0.312	0.256	0.239	0.248	0.097
0	0.155	0.147	0.151