Human Interferon α,IFN-α ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E08636h）為雙抗夾心法ELISA試劑盒，定量檢測樣本中的IFN-α含量。IFN-α即α-干擾素，是一類具有多種生物活性的細胞因子。背景上，它在抗病毒、抗腫瘤及免疫調(diào)節(jié)等方面有重要作用。研究機制方面，它能與細胞表面受體結(jié)合，激活相關(guān)信號通路，誘導(dǎo)多種抗病毒蛋白產(chǎn)生，抑制病毒復(fù)制，還可調(diào)節(jié)免疫細胞功能。科研人員可通過精準(zhǔn)分析病毒感染模型中的免疫激活狀態(tài)、評估免疫調(diào)節(jié)藥物對IFN-α分泌的影響，或探究腫瘤免疫治療過程中的細胞因子動態(tài)變化。為免疫機制研究及生物制劑開發(fā)提供高效檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
IFL ELISA Kit; IFN alpha ELISA Kit; IFN alpha 1/13 ELISA Kit; IFN alpha 1b ELISA Kit; IFN alphaD ELISA Kit; IFN ELISA Kit; IFN-alpha-1/13 ELISA Kit; IFNA@ ELISA Kit; IFNA1 ELISA Kit; IFNA1_HUMAN ELISA Kit; IFNA13 ELISA Kit; Interferon alpha 1 ELISA Kit; Interferon alpha-1/13 ELISA Kit; Interferon alpha-D ELISA Kit; Interferon alpha1 ELISA Kit; Interferon, alpha 13 ELISA Kit; LeIF D ELISA Kit
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縮寫：
IFN-α
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, cell culture supernates, urine, tissue homogenates
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檢測范圍：
7.8 pg/ml-500 pg/ml
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靈敏度：
1.95 pg/ml
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Immunology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
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線性度：
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回收率：
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標(biāo)準(zhǔn)曲線：
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IFN-αantibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IFN-α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻
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LncRNA MALAT1 facilitates HIV-1 replication by upregulation of CHCHD2 and downregulation of IFN-I expression
MR Wang, CS Bai, J Dai, L Yang, FY Quan, J Ma,Molecular & Cellular Proteomics,2025
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Особенности цитокиновой продукции у пациентов с бактериальной пневмонией, протекающей на фоне острой респираторной вирусной инфекции
ВК Парфенюк, НВ Бондарь,Российский медико-биологический вестник имени академика ИП Павлова,2025
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Heterozygous de novo dominant negative mutation of REXO2 results in interferonopathy
E Idiiatullina, M Al-Azab, M Lin,Nature Communications,2024
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M2 macrophage exosomal LINC01001 promotes non-small cell lung cancer development by affecting METTL3 and glycolysis pathway
L Xu,Cancer gene therapy,2023
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The mechanism of Langchuangding in treatment of systemic lupus erythematosus via modulating TLR7-IRF7-IFNα pathway
Q Zhang,Heliyon,2024
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NLRX1 can counteract innate immune response induced by an external stimulus favoring HBV infection by competitive inhibition of MAVS-RLRs signaling in HepG2-NTCP cells
Q Jiao,Science progress,2021
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β-adrenergic Receptor Inhibition Enhances Oncolytic Herpes Virus Propagation Through STAT3 Activation in Gastric Cancer
J Hu,/,2021
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Downregulation of miR-155-5p facilitates enterovirus 71 replication through suppression of type I IFN response by targeting FOXO3/IRF7 pathway
Yang D,Cell Cycle,2019
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Interferon alpha, gamma, and lambda 2: are they possible culprits in the association between psoriasis and hepatitis C virus?
Randa Youssef,Journal of The Egyptian Women\'s Dermatologic Society,2019
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Increased interferon-1α, interleukin-10 and BLyS concentrations as clinical activity biomarkers in systemic lupus erythematosus
Fernandez Matilla M, et al,Medicina Clinica,2019
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A novel engineered interferon-α hybrid molecule increases anticancer efficacy of doxorubicin in breast cancer chemotherapy
Li SJ.et al,Int J Oncol.,2017
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Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow.
Xu Z.et al,Int J Clin Exp Med.,2015
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