Rat Interferon α,IFN-α ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠α干擾素(IFN-α)酶聯免疫試劑盒（CSB-E08637r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IFNA1含量。IFNA1即干擾素α1，是Ⅰ型干擾素家族成員。它具有抗病毒、免疫調節等功能。其研究機制主要是通過與細胞表面受體結合，激活下游信號通路，誘導多種抗病毒蛋白表達，從而發揮對病毒復制和傳播的抑制作用，在抗病毒及免疫相關研究中受關注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外免疫學研究、病毒感染模型評估或藥物干預實驗中IFN-α表達水平的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit
縮寫：

IFN-α
Uniprot No.：

P05011
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-91
1:2	Average %	92
	Range %	85-97
1:4	Average %	99
	Range %	94-104
1:8	Average %	103
	Range %	99-108

回收率：

The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亞細胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數據庫鏈接：

KEGG: rno:298210

STRING: 10116.ENSRNOP00000051043

UniGene: Rn.196548