Human Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2) ELISA kit
-
中文名稱：人胰島素樣生長(zhǎng)因子2 mRNA結(jié)合蛋白2(IGF2BP2)酶聯(lián)免疫試劑盒
-
貨號(hào)：CSB-EL011091HU
-
規(guī)格：96T/48T
-
價(jià)格：￥3600/￥2500
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：人胰島素樣生長(zhǎng)因子2 mRNA結(jié)合蛋白2(IGF2BP2)酶聯(lián)免疫試劑盒（CSB-EL011091HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的IGF2BP2含量。IGF2BP2 是一種 RNA 結(jié)合蛋白。它可通過(guò)與特定 RNA 結(jié)合，調(diào)控基因表達(dá)和細(xì)胞活動(dòng)。研究發(fā)現(xiàn)其與腫瘤發(fā)生發(fā)展有關(guān)，在肝癌、乳腺癌等癌癥中表達(dá)異常，影響細(xì)胞增殖、凋亡等過(guò)程，有望成為癌癥診斷標(biāo)志物和治療靶點(diǎn)。試劑盒檢測(cè)范圍為125 pg/mL-8000 pg/mL，適用于體外細(xì)胞模型構(gòu)建、疾病動(dòng)物模型評(píng)估以及臨床前樣本的分子標(biāo)志物探索等科研場(chǎng)景，助力于腫瘤微環(huán)境研究、代謝性疾病機(jī)制解析及相關(guān)藥物開(kāi)發(fā)等基礎(chǔ)研究領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
-
別名：Hepatocellular carcinoma autoantigen p62 ELISA Kit; IF2B2_HUMAN ELISA Kit; IGF 2 mRNA binding protein 2 ELISA Kit; IGF II mRNA binding protein 2 ELISA Kit; IGF-II mRNA-binding protein 2 ELISA Kit; IGF2 mRNA-binding protein 2 ELISA Kit; Igf2bp2 ELISA Kit; IMP 2 ELISA Kit; IMP-2 ELISA Kit; IMP2 ELISA Kit; Insulin like growth factor 2 mRNA binding protein 2 ELISA Kit; Insulin-like growth factor 2 mRNA-binding protein 2 ELISA Kit; OTTHUMP00000082606 ELISA Kit; OTTHUMP00000082607 ELISA Kit; OTTHUMP00000082608 ELISA Kit; p62 ELISA Kit; VICKZ 2 ELISA Kit; VICKZ family member 2 ELISA Kit; VICKZ2 ELISA Kit
-
縮寫(xiě)：
-
Uniprot No.：
-
種屬：Homo sapiens (Human)
-
樣本類型：serum, plasma, tissue homogenates, cell lysates
-
檢測(cè)范圍：125 pg/mL-8000 pg/mL
-
靈敏度：31.25 pg/mL
-
反應(yīng)時(shí)間：1-5h
-
樣本體積：50-100ul
-
檢測(cè)波長(zhǎng)：450 nm
-
研究領(lǐng)域：Epigenetics and Nuclear Signaling
-
測(cè)定原理：quantitative
-
測(cè)定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-90 1:2 Average % 96 Range % 92-100 1:4 Average % 90 Range % 85-94 1:8 Average % 104 Range % 100-108 -
回收率：
The recovery of human IGF2BP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 84-93 EDTA plasma (n=4) 95 90-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 8000 2.167 2.249 2.208 2.051 4000 1.649 1.703 1.676 1.519 2000 1.174 1.114 1.144 0.987 1000 0.791 0.758 0.775 0.618 500 0.458 0.472 0.465 0.308 250 0.335 0.347 0.341 0.184 125 0.226 0.236 0.231 0.074 0 0.153 0.160 0.157 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻(xiàn)
- The m6A reader IGF2BP2 regulates glycolytic metabolism and mediates histone lactylation to enhance hepatic stellate cell activation and liver fibrosis Y Zhou,Cell death & disease,2024
- The m6A reader IGF2BP2 regulates glycolytic metabolism and mediates histone lactylation to enhance hepatic stellate cell activation and liver fibrosis X Li,researchsquare,2023
- Interplay of endothelial-mesenchymal transition inflammation and autophagy in proliferative diabetic retinopathy pathogenesis K Himaja,Heliyon,2024
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能：RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.
-
基因功能參考文獻(xiàn)：
- IGF2BP2 protein is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1. PMID: 28753127
- The miR-485-5p/IGF2BP2 axis orchestrates the growth and metastasis of non-small cell lung cancer. PMID: 29510198
- IGF2BP2/IMP2 expression is linked to short survival and metastasis in esophageal adenocarcinoma PMID: 27391348
- insulin-like growth factor 2 mRNA-binding protein 2 (IMP2) binds to let-7 miRNA recognition elements (MREs) and prevents let-7 target gene silencing. PMID: 27184842
- miR-1193 suppressed proliferation and invasion of human breast cancer cells via translational suppression of IGF2BP2. PMID: 27733218
- Our data suggests that IGF2BP2 polymorphisms are associated with Type 2 Diabetes Mellitus in Asian populations. PMID: 27294943
- Study conclude that integration of genomic and transcriptomic data implicate circulating IGF2BP2 mRNA levels associated with glucose and insulin homeostasis PMID: 27625022
- IGF2BP2 as a post-transcriptional regulatory mRNA-binding factor that contributes to Colorectal carcinogenesis. PMID: 27153315
- p62/IMP2 stimulates cell migration and reduces cell adhesion, contributing to breast cancer progression. PMID: 26416451
- IMP2 expression is higher in ovarian and endometrial high-grade serous carcinomas (HGSC) than in ovarian or endometrial endometrioid carcinoma. Knockdown in ovarian HGSC cell line decreased cell proliferation. PMID: 26889980
- Our results suggest IGF2BP2 and KCNQ1 polymorphisms might be independent predictors of chemotherapeutic response PMID: 26115082
- HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion PMID: 26107517
- Imp2 regulates the activity of IGF2, which further activates PI3K/Akt signaling. PMID: 25719943
- The IGF2BP2 gene rs1470579 and rs4402960 polymorphisms were associated with type 2 diabetes patients and therapeutic efficacy of pioglitazone in this Chinese population PMID: 25247335
- The IGF2BP2 gene rs4402960 polymorphism increases the breast cancer risk of Chinese females with Han nationality, and is a breast cancer predisposing gene. PMID: 25661373
- rs4402960 of IGF2BP2 gene is a strong candidate for Type 2 diabetes susceptibility and overweight/obesity risk. PMID: 24636221
- Data suggest that autoantibody against IGF2 mRNA-Binding Protein 2 (IMP2/p62) may be a useful serum biomarker for early-stage breast cancer screening and diagnosis. PMID: 25721883
- The case-control study and meta-analysis revealed a significant association between the IGF2BP2 rs4402960 variant and type 2 diabetes in Moroccan and Arab populations.[meta-analysis] PMID: 24898818
- The present study provided data suggesting that the wild C allele of IGF2BP2 (rs4402960) had a protective effect against T2DM in obese subjects of Chinese Han population. PMID: 25062844
- VICKZ1 and VICKZ2 are overexpressed in ovarian carcinoma effusions suggesting a biological role at this anatomical site, and appear to have a role in proteolysis and invasion. VICKZ2 may be a prognostic marker in ovarian serous carcinoma effusions. PMID: 24814803
- IGF2BP2 was strongly associated with the risk of T2DM in Chinese Han population. PMID: 24229666
- Our results revealed two novel genes (IGF2BP2 and TNFRSF13B), whose function could account for the biologic pathways influencing MetS phenotypes. PMID: 23418049
- Polymorphisms in PPARgamma(2) and IGF2BP2 were shown to be highly correlated with GDM occurrence, whereas no correlation was found for KCNQ1 polymorphisms. PMID: 23364967
- TCF7L2 was replicated in this study (P = 0.004; combined analysis P = 3.8 x 10(-6)), and type 2 diabetes SNPs at or near CDKAL1, CDKN2A/B, and IGF2BP2 were associated with CFRD PMID: 23670970
- IGF2BP2 genetic variants contribute to insulin resistance in Russian NIDDM patients. PMID: 23403707
- findings implicate the HMGA2-IGFBP2-NRAS signaling pathway as a critical oncogenic driver in embryonic rhabdomyosarcoma PMID: 23536553
- IGF2BP2 may play a role in susceptibility to schizophrenia in Han Chinese, supporting the hypothesis that the co-occurrence of type 2 diabetes mellitus and schizophrenia may be explained by shared genetic risk variants. PMID: 23656854
- rs4402960 and rs1470579 lymorphisms of the IFG2BP2po is a risk factor for developing type 2 diabetes. PMID: 22015911
- there is a strong immune response of anti-p62 in sera from patients with colon cancer compared with normal human sera (NHS). PMID: 23421499
- p62 exerts IGF2-independent antiapoptotic action, which is facilitated via phosphorylation of ERK1/2. PMID: 23257922
- IGF2BP2 alternative variants were associated with GADA negative diabetes. The IGF2BP2 haplotypes and diplotypes increased the risk of diabetes in Malaysian subject. PMID: 23029108
- In African Americans, seven of the 29 SNPs examined were found to be associated with T2D risk at P PMID: 23144361
- genetic association studies: Data suggest that an SNP in IGF2BP2 (rs4402960) is associated with type 2 diabetes; IGF2BP2 may have genetic interactions with insulin-like growth factor II with a protective effect in male patients with type 1 diabetes. PMID: 22770937
- Association to type 2 diabetes was found for rs13266634 (SLC30A8), rs7923837 (HHEX), rs10811661 (CDKN2A/2B), rs4402960 (IGF2BP2), rs12779790 (CDC123/CAMK1D), and rs2237892 (KCNQ1). PMID: 22923468
- oncofetal insulin-like growth factor 2 mRNA-binding protein 2 (IMP2, IGF2BP2) regulates oxidative phosphorylation (OXPHOS) in primary glioblastoma (GBM) sphere cultures (gliomaspheres) PMID: 22899010
- Data validate that IGF2BP2 susceptibility variants rs4402960 and rs1470579 associate with T2DM in Lebanese Arabs. PMID: 22245690
- Two isoforms of the mRNA binding protein IGF2BP2 are generated by alternative translational initiation. PMID: 22427968
- IGF2BP2 genetic variation is associated with type 2 diabetes. PMID: 22032244
- Six SNP(rs7754840 in CDKAL1, rs391300 in SRR, rs2383208 in CDKN2A/2B, rs4402960 in IGF2BP2, rs10830963 in MTNR1B, rs4607517 in GCK)risk alleles of type 2 diabetes were associated with GDM in pregnant Chinese women. PMID: 22096510
- meta-analysis suggested that IGF2BP2 rs4402960 polymorphism conferred elevated risk of T2DM, especially in European, East Asian and South Asian populations PMID: 21839790
- involved in the selective autophagic clearance of non-ubiquitylated aggregation-prone substrates PMID: 21771882
- IGF2 is emerging as an important gene for ovarian cancer. PMID: 21422097
- The induction of a steatotic phenotype implies that p62 plays a role in hepatic pathophysiology PMID: 21145819
- Double phosphorylation promotes IMP2 binding to the IGF2 leader 3 mRNA 5' untranslated region, and the translational initiation of this mRNA PMID: 21576258
- non-replications of IGF2BP2 associations with type 2 diabetes PMID: 20627640
- Data reveal how the posttranscriptional regulation of gene expression by IMP-2 contributes to the control of adhesion structures and stable microtubules and demonstrate an important function for IMP-2 in cellular motility. PMID: 20956565
- findings show that IGF2BP2 rs1470579 and rs4402960 polymorphisms may be associated with the development of type 2 diabetes and these polymorphisms may affect the therapeutic efficacy of repaglinide in Chinese T2DM patientsmellitus PMID: 20523342
- prostate cancer was inversely associated with the IGF2BP2 rs4402960 T allele PMID: 20142250
- HMGA2 differentially regulates expression of IMP family members during mouse embryogenesis. PMID: 15225648
- Study suggests that there is a significant association between expression of IMP-2 and the growth of tumor cells, in which IMP-2 is associated with apoptosis induced by tretinoin. PMID: 15618018
顯示更多
收起更多
-
亞細(xì)胞定位：Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules.
-
蛋白家族：RRM IMP/VICKZ family
-
組織特異性：Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, ki
-
數(shù)據(jù)庫(kù)鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
