Human Hyaluronidase PH-20(SPAM1) ELISA kit

Human Hyaluronidase PH-20(SPAM1) ELISA kit

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中文名稱：

人透明質酸酶PH-20(SPAM1)酶聯免疫試劑盒
貨號：

CSB-EL022471HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人透明質酸酶PH-20(SPAM1)酶聯免疫試劑盒（CSB-EL022471HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的SPAM1含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產品適用于基礎科研中對PH - 20的定量分析，可用于探究生殖生物學中的受精機制、腫瘤侵襲轉移中的基質重塑作用，或評估疾病模型中透明質酸代謝相關通路的調控機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit
縮寫：

SPAM1
Uniprot No.：

P38567
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	97
	Range %	91-102
1:4	Average %	90
	Range %	85-94
1:8	Average %	85
	Range %	80-89

回收率：

The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	87	82-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.374	2.452	2.413	2.313
500	1.619	1.676	1.648	1.548
250	0.936	1.004	0.970	0.870
125	0.537	0.549	0.543	0.443
62.5	0.381	0.374	0.378	0.278
31.2	0.257	0.259	0.258	0.158
15.6	0.168	0.164	0.166	0.066
0	0.102	0.098	0.100

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

VCN-01 disrupts pancreatic cancer stroma and exerts antitumor effects M Bazan-Peregrin,The Journal for ImmunoTherapy of Cancer,2021
E cient Production of Human Hyaluronidase PH20 in HEK293T Cells Using Site Specific Integration K Dormiani,/,2021

功能：

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
基因功能參考文獻：
1. Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID: 25209425
2. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID: 24778442
3. HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID: 23247813
4. The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID: 23209833
5. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
6. SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID: 12932297
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Glycosyl hydrolase 56 family
組織特異性：

Testis.
數據庫鏈接：

HGNC: 11217

OMIM: 600930

KEGG: hsa:6677

STRING: 9606.ENSP00000345849

UniGene: Hs.121494

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.