Human Glypican-4(GPC4) ELISA kit

Human Glypican-4(GPC4) ELISA kit

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中文名稱：

人磷脂酰肌醇聚糖4(GPC4)酶聯免疫試劑盒
貨號：

CSB-EL009706HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人磷脂酰肌醇聚糖4(GPC4)酶聯免疫試劑盒（CSB-EL009706HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的GPC4含量。GPC4，全稱Glypican 4，是一種位于Xq26.2染色體上的基因，其編碼的蛋白質屬于細胞表面硫酸乙酰肝素蛋白聚糖家族。該基因在細胞分裂和生長調節中發揮作用，可能與Simpson-Golabi-Behmel綜合征有關。研究機制方面，GPC4基因的表達與多種生物學過程相關，包括細胞信號傳導、細胞增殖和腫瘤發生等。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎科研領域中對GPC4蛋白的功能機制研究，例如腫瘤微環境分析、干細胞特性探索、胚胎發育相關信號通路研究等場景，為體外實驗提供穩定可靠的檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

dJ900E8.1 glypican 4 ELISA Kit; Glypican proteoglycan 4 ELISA Kit; Gpc4 ELISA Kit; GPC4_HUMAN ELISA Kit; K Glypican ELISA Kit; K-glypican ELISA Kit; Kglypican ELISA Kit; OTTHUMP00000024052 ELISA Kit; Secreted glypican 4 ELISA Kit; Secreted glypican-4 ELISA Kit; UNQ474/PRO937 ELISA Kit
縮寫：

GPC4
Uniprot No.：

O75487
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	92
	Range %	88-96
1:4	Average %	87
	Range %	81-94
1:8	Average %	98
	Range %	92-106

回收率：

The recovery of human GPC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	95	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.254	2.355	2.305	2.163
20	1.428	1.528	1.478	1.336
10	0.935	0.956	0.946	0.804
5	0.556	0.567	0.562	0.420
2.5	0.372	0.361	0.367	0.225
1.25	0.268	0.257	0.263	0.121
0.625	0.215	0.212	0.214	0.072
0	0.142	0.141	0.142

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
基因功能參考文獻：
1. The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID: 27071854
2. According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID: 27425250
3. serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID: 26486309
4. Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID: 23633195
5. new function of miR-125a by targeting gene glypican-4 in cell growth process PMID: 22644326
6. Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID: 22761013
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-4]: Secreted, extracellular space.
蛋白家族：

Glypican family
數據庫鏈接：

HGNC: 4452

OMIM: 300168

KEGG: hsa:2239

STRING: 9606.ENSP00000359864

UniGene: Hs.58367

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.