Human Fibroblast growth factor 5(FGF5) ELISA kit

Human Fibroblast growth factor 5(FGF5) ELISA kit

產品詳情

引用文獻

產品評價

靶點詳情

產品詳情

引用文獻

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息

文獻引用 (1) 說明書下載

中文名稱：

人成纖維細胞生長因子5(FGF5)酶聯免疫試劑盒
貨號：

CSB-EL008632HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品描述：

人成纖維細胞生長因子5(FGF5)酶聯免疫試劑盒（CSB-EL008632HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF5含量。FGF5（成纖維細胞生長因子5）是一種分泌信號蛋白，主要在間充質和骨骼肌中表達。它對哺乳動物毛發周期性生長起關鍵作用，同時與動物肢體發育及人類多形性膠質母細胞瘤、黑色素瘤、肝細胞癌和高血壓有關。研究顯示，FGF5通過調節細胞增殖和信號通路影響這些過程。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外研究場景，如探究FGF5在毛囊生物學、皮膚病理模型或組織工程中的功能機制，亦可應用于藥物開發中靶向分子效應的評估。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FGF 5 ELISA Kit; FGF-5 ELISA Kit; FGF5 ELISA Kit; FGF5_HUMAN ELISA Kit; Fibroblast growth factor 5 ELISA Kit; HBGF 5 ELISA Kit; HBGF-5 ELISA Kit; heparin binding growth factor 5 ELISA Kit; Heparin-binding growth factor 5 ELISA Kit; Smag 82 ELISA Kit; Smag-82 ELISA Kit; TCMGLY ELISA Kit
縮寫：

FGF5
Uniprot No.：

P12034
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	106
	Range %	100-113

回收率：

The recovery of human FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.314	2.227	2.271	2.126
500	1.816	1.876	1.846	1.701
250	1.334	1.402	1.368	1.223
125	0.801	0.840	0.821	0.676
62.5	0.544	0.518	0.531	0.386
31.2	0.372	0.359	0.366	0.221
15.6	0.271	0.282	0.277	0.132
0	0.142	0.147	0.145

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway F Chen,Journal of cellular and molecular medicine,2023

功能：

Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
基因功能參考文獻：
1. Study indicated that MTHFR rs1801133, FGF5 rs16998073 and CSK rs1378942 were associated with increased the risk of obesity in the Chinese children. PMID: 30217759
2. FGF5 is an independent protective factor for breast cancer patients. PMID: 29804124
3. These results provide new insight into the functions of miR-9-3p and HBGF-5 in HCC and identify miR-9-3p as a potential therapeutic target for HCC PMID: 28750499
4. FGF5 facilitates cell proliferation through ERK1/2 activation, and it influences the osteogenic differentiation of tonsil-derived mesenchymal stem cells. PMID: 27224250
5. These findings collectively demonstrate a tumor suppressor role of miR-188-5p in HCC progression via targeting FGF5 PMID: 25998163
6. High BMI increases the effect of the blood pressure-increasing allele at rs1458038 near FGF5 in a Chinese population. PMID: 25618516
7. In Chinese children, no association of CSK rs1378942, MTHFR rs1801133, CYP17A1 rs1004467, STK39 rs3754777 and FGF5 rs16998073 with BP/risk of hypertension. PMID: 23759979
8. FGF5 is a crucial regulator of hair growth in humans. PMID: 24989505
9. Meta-analysis indicated significant associations of both CYP17A1 rs11191548 and FGF5 rs16998073 polymorphisms with hypertension susceptibility in East Asians. PMID: 22959498
10. Variants in or near FGF5, CYP17A1 and MTHFR contributed to variation in BP and hypertension risk. Effect sizes of these three loci tended to be larger in Chinese than in white Europeans PMID: 20852445
11. variation in FGF5 and ZNF652 gene upstream regions with altered susceptibility to hypertension in Han Chinese PMID: 20542020
12. The role of the proteasome is confirmed when a spliced FGF-5 peptide is produced in vitro after incubation of proteasomes with a 49-amino-acid precursor peptide in a transpeptidation splicing model. PMID: 20154207
13. FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects. PMID: 18362893
顯示更多

收起更多
相關疾病：

Trichomegaly (TCMGLY)
亞細胞定位：

Secreted.
蛋白家族：

Heparin-binding growth factors family
組織特異性：

Expressed in neonatal brain.
數據庫鏈接：

HGNC: 3683

OMIM: 165190

KEGG: hsa:2250

STRING: 9606.ENSP00000311697

UniGene: Hs.37055

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.