Human Fibrillin-3(FBN3) ELISA kit

Human Fibrillin-3(FBN3) ELISA kit

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中文名稱：

人原纖維蛋白-3(FBN3)酶聯免疫試劑盒
貨號：

CSB-EL008458HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人原纖維蛋白-3(FBN3)酶聯免疫試劑盒（CSB-EL008458HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FBN3含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品可應用于心血管發育、結締組織相關疾病模型或遺傳學研究中FBN3蛋白表達的動態監測，為探索其生物學功能及調控機制提供工具支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FBN3 ELISA Kit; KIAA1776Fibrillin-3 [Cleaved into: Fibrillin-3 C-terminal peptide] ELISA Kit
縮寫：

FBN3
Uniprot No.：

Q75N90
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FBN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	94
	Range %	89-98
1:4	Average %	91
	Range %	88-98
1:8	Average %	86
	Range %	80-96

回收率：

The recovery of human FBN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	81-93
EDTA plasma (n=4)	98	95-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.409	2.456	2.433	2.325
10	1.535	1.585	1.560	1.452
5	0.812	0.801	0.807	0.699
2.5	0.506	0.545	0.526	0.418
1.25	0.272	0.265	0.269	0.161
0.625	0.166	0.155	0.161	0.053
0.312	0.150	0.158	0.154	0.046
0	0.104	0.111	0.108

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-containing microfibrils provide long-term force bearing structural support.
基因功能參考文獻：
1. an association study showed a potential association of the D19S884 marker with PCOS in Chinese Han women and the meta-analysis identified that the A8 allele may increase susceptibility to PCOS PMID: 23265956
2. The FBN3 risk allele may be associated with changes in basal glucose homeostasis in PCOS. PMID: 22301903
3. Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746
4. fibrillin-3, compared to the other fibrillins, fulfills both overlapping and distinct functions in human development PMID: 20970500
5. A variant in the fibrillin-3 gene is associated with TGF-beta and inhibin B levels in women with polycystic ovary syndrome. PMID: 20630504
6. Loss of fibrillin-3 during folliculogenesis may be an important factor in polycystic ovary syndrome pathogenesis. PMID: 20855553
7. Single nucleotide polymorphisms in FBN3 occur and may be a candidate gene for polycystic ovary syndrome. PMID: 20200332
8. Data show that fibrillin 3 appears to have little involvement in PCOS but cannot rule out that other markers in the region of chromosome 19p13.2 are associated with PCOS or that FBN3 expression occurs in other organs that may influence the PCOS phenotype. PMID: 19692420
9. fibrillin-3 gene showed the strongest evidence for association with Polycystic ovary syndrome PMID: 17785364
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibrillin family
組織特異性：

Predominantly expressed in connective tissues such as skeletal muscle, tendon, skin, perichondrium and periosteum. Highly expressed in fetal lung, brain, kidney. Expressed at low level in prostate, testis, mammary gland, uterus, ovary, placenta, bladder,
數據庫鏈接：

HGNC: 18794

OMIM: 608529

KEGG: hsa:84467

STRING: 9606.ENSP00000270509

UniGene: Hs.370362

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.