Human Endothelin-2(EDN2)ELISA Kit

說明書下載

中文名稱：

人內皮素2(EDN2)酶聯免疫試劑盒
貨號：

CSB-E16518h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人內皮素2(EDN2)酶聯免疫試劑盒（CSB-E16518h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EDN2含量。試劑盒檢測范圍為4.688 pg/mL-300 pg/mL，該產品適用于基礎科研領域，可用于研究EDN2在生殖生理、心血管調節或腫瘤微環境中的表達規律，為探索其分子機制、構建疾病模型或評估藥物干預效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

EDN2 ELISA Kit; EDN2_HUMAN ELISA Kit; Endothelin 2 ELISA Kit; Endothelin-2 ELISA Kit; ET 2 ELISA Kit; ET-2 ELISA Kit; ET2 ELISA Kit; PPET2 ELISA Kit; Preproendothelin 2 ELISA Kit; Preproendothelin-2 ELISA Kit
縮寫：

EDN2
Uniprot No.：

P20800
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.688 pg/mL-300 pg/mL
靈敏度：

1.172 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	86
	Range %	81-92
1:4	Average %	94
	Range %	87-98
1:8	Average %	86
	Range %	80-91

回收率：

The recovery of human EDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	1.832	1.988	1.910	1.802
150	1.214	1.192	1.203	1.095
75	0.760	0.721	0.741	0.633
37.5	0.442	0.451	0.447	0.339
18.75	0.287	0.276	0.282	0.174
9.375	0.212	0.217	0.215	0.107
4.688	0.155	0.160	0.158	0.050
0	0.107	0.108	0.108

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Endothelins are endothelium-derived vasoconstrictor peptides.
基因功能參考文獻：
1. EDN2 expression in left internal mammary arteries depends on tissue harvesting technique. PMID: 24647318
2. Overexpression of Edn2 in the mouse retina causes an excess of tip cells and inhibits vascular growth. Overexpression of Edn2 in the brain and skin had no effect, but vascular development was inhibited in midgestation mouse embryos. PMID: 24043815
3. Endothelin 2 and endothelin receptor type B may be a regenerative pathway for central nervous system myelination and remyelination. PMID: 23518706
4. Studies revealed hypermethylation of EDN2 and EDN3 genes in human primary colon cancers and in a panel of human colon cancer cell lines. Epigenetic inactivation of ET-2 and ET-3 occurs frequently in both rat and human colon cancers. PMID: 22865632
5. plasma levels of big ET-2, and big ET-3 are markedly increased in patients with end stage renal disease on hemodialysis PMID: 22921304
6. In granulosa lutein cells from women with polycystic ovary syndrome, endothelin-2 mRNA was reduced as compared with normally ovulating women PMID: 22727793
7. although endothelin 2 does not appear to be an independent prognostic marker, there is evidence of a putative role in clear cell renal cell carcinoma progression PMID: 22047647
8. Endothelin-2 is a macrophage chemoattractant. ET-2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia. PMID: 12207323
9. Endothelin-2 is a hypoxia-induced autocrine survival factor for breast tumor cells PMID: 12516960
10. The EDN2 A985 allele, which is considered to be protective in cardiovascular disease, may be a risk factor for atrial fibrillation in patients with hypertrophic cardiomyopathy. PMID: 18037749
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亞細胞定位：

Secreted.
蛋白家族：

Endothelin/sarafotoxin family
組織特異性：

Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
數據庫鏈接：

HGNC: 3177

OMIM: 131241

KEGG: hsa:1907

STRING: 9606.ENSP00000361668

UniGene: Hs.1407

Human Endothelin-2(EDN2)ELISA Kit

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