Human Complement component C8 beta chain(C8B) ELISA kit

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產品詳情

產品描述：

人補體成分C8β鏈(C8B)酶聯免疫試劑盒（CSB-EL004195HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C8B含量。人補體成分C8β鏈（C8B）是補體系統的一部分，參與免疫反應和炎癥過程。其研究機制涉及C8β鏈與C5b67結合，激活C8αγ，進而參與膜攻擊復合物的形成，對病原體進行殺傷。試劑盒檢測范圍為2.74 pg/mL-2000 pg/mL，其標準化操作流程支持科研人員探究補體系統在感染、炎癥或自身免疫性疾病中的作用機制，例如通過分析不同病理條件下血清或組織中C8β鏈的表達差異，揭示補體激活途徑的調控規律。此外，還可用于評估藥物干預或基因編輯模型對補體相關通路的影響，為免疫學基礎研究及轉化醫學提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C8b ELISA Kit; CO8B_HUMAN ELISA Kit; Complement component 8 beta polypeptide ELISA Kit; Complement component 8 subunit beta ELISA Kit; Complement component C8 beta chain ELISA Kit
縮寫：

C8B
Uniprot No.：

P07358
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.74 pg/mL-2000 pg/mL
靈敏度：

1.37 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10000	Average %	92
	Range %	89-98
1:20000	Average %	90
	Range %	84-100
1:40000	Average %	95
	Range %	92-101
1:80000	Average %	93
	Range %	86-98

回收率：

The recovery of human C8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-104
EDTA plasma (n=4)	91	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
基因功能參考文獻：
1. The binding specificity between C8 beta and C8 alpha subunits is determined by a cooperative interaction of the N-terminal thrombospondin type 1 module and the membrane attack complex/perforin domain. PMID: 12220191
2. results indicate that the principal binding site for C9 lies within the MACPF domain of C8alpha; they also suggest this site and the binding sites for C8beta and C8gamma are distinct PMID: 16618117
相關疾病：

Complement component 8 deficiency, 2 (C8D2)
亞細胞定位：

Secreted.
蛋白家族：

Complement C6/C7/C8/C9 family
數據庫鏈接：

HGNC: 1353

OMIM: 120960

KEGG: hsa:732

STRING: 9606.ENSP00000360281

UniGene: Hs.391835