Human Complement Fragment 3d,C3d ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人補(bǔ)體片段3d(C3d)酶聯(lián)免疫試劑盒（CSB-E14213h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3d含量。C3d是補(bǔ)體系統(tǒng)C3的裂解片段。在研究機(jī)制方面，它可作為補(bǔ)體激活的標(biāo)志物，也參與免疫應(yīng)答的調(diào)節(jié)。其與B細(xì)胞表面的CD21相互作用，能增強(qiáng)B細(xì)胞對相關(guān)抗原的免疫反應(yīng)，在感染、自身免疫病等研究領(lǐng)域有重要意義。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，適用于基礎(chǔ)科研中補(bǔ)體激活程度的動態(tài)監(jiān)測、自身免疫性疾病模型的分子機(jī)制解析，以及感染或炎癥條件下補(bǔ)體旁路活化的相關(guān)性研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C3d
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C3d in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	89-98
1:10	Average %	90
	Range %	87-98
1:20	Average %	94
	Range %	90-98
1:40	Average %	93
	Range %	89-97

回收率：

The recovery of human C3d spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.