Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit

產品詳情

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文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Ⅱ型膠原(Col Ⅱ)酶聯免疫試劑盒（CSB-E08087h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅱ含量。Col Ⅱ，即II型膠原蛋白，是構成透明軟骨的基礎，由膠原蛋白II型α1鏈的同源三聚體組成。它在軟骨中占比高達50%，負責提供組織的拉伸強度和蛋白聚糖的捕獲。在研究中，Col Ⅱ的合成和分泌在體外培養的軟骨細胞中持續存在，但其合成在細胞快速增殖時會被關閉，這為組織工程軟骨提供了新的策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外科研實驗中定量分析Col Ⅱ的表達動態，例如研究軟骨組織工程材料的生物相容性、關節炎模型中基質代謝變化、軟骨細胞分化調控機制等。為軟骨相關疾病機制探索、藥物干預效果評估等基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
縮寫：

Col Ⅱ
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-90
1:200	Average %	93
	Range %	88-98
1:400	Average %	94
	Range %	90-100
1:800	Average %	96
	Range %	90-102

回收率：

The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.