Human Cartilage intermediate layer protein 2(CILP2) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人軟骨間層蛋白2(CILP2)酶聯免疫試劑盒（CSB-EL005438HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CILP2含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，本試劑盒通過高特異性配對抗體實現精準捕獲與信號放大，適用于軟骨相關疾病模型研究、藥物干預效果評估以及生物標志物篩選等科研場景。支持多種生物樣本類型檢測，實驗操作全程無需特殊設備，配套標準品與預包被板條可滿足實驗室常規檢測需求，為軟骨生物學、運動醫學及再生醫學領域的基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CILP2 ELISA Kit; Cartilage intermediate layer protein 2 ELISA Kit; CILP-2) [Cleaved into: Cartilage intermediate layer protein 2 C1; Cartilage intermediate layer protein 2 C2] ELISA Kit
縮寫：

CILP2
Uniprot No.：

Q8IUL8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CILP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-103
1:2	Average %	96
	Range %	90-100
1:4	Average %	85
	Range %	80-90
1:8	Average %	92
	Range %	87-98

回收率：

The recovery of human CILP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-93
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

May play a role in cartilage scaffolding.
基因功能參考文獻：
1. This study aims to analyze the influences of single-nucleotide polymorphism in the NCAN-CILP2 region on non-alcoholic fatty liver disease and plasma lipid levels in the Asian and Pacific ethnic groups. PMID: 26758378
2. The minor T allele of CILP2 gene and I allele of ACE gene have a protective effect. PMID: 24350279
3. Cartilage intermediate layer protein 2 (CILP-2) is expressed in articular and meniscal cartilage and down-regulated in experimental osteoarthritis. PMID: 21880736
4. Data show no differences in triglyceride or total cholesterol levels in relation to any allelic variants of ANGPTL3, CILP2, or TRIB1 SNPs. PMID: 21691831
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Expressed in articular chondrocytes but not in knee meniscal cartilage cells. Localizes to the intermediate to deep zone of articular cartilage.
數據庫鏈接：

HGNC: 24213

OMIM: 612419

KEGG: hsa:148113

STRING: 9606.ENSP00000291495

UniGene: Hs.279574