Human C-type lectin domain family 2 member D(CLEC2D) ELISA kit

Human C-type lectin domain family 2 member D(CLEC2D) ELISA kit

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中文名稱：

人C型凝集素結構域家族2成員D(CLEC2D)酶聯免疫試劑盒
貨號：

CSB-EL005528HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人C型凝集素結構域家族2成員D(CLEC2D)酶聯免疫試劑盒（CSB-EL005528HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CLEC2D含量。CLEC2D作為靶點，在人體生理疾病過程中可能有重要作用。目前相關研究或聚焦于其在免疫調節、細胞信號傳導等機制方面。深入探究其背景及作用機制，能為開發針對性治療方法、藥物等提供理論支撐，助力攻克相關疾病難題。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于探索CLEC2D在免疫調節、腫瘤微環境、感染性疾病或自身免疫性疾病中的分子機制研究，為體外實驗提供可靠的定量數據支持。適用于基礎科研中細胞模型、動物模型或臨床前樣本的分析，為研究CLEC2D的功能及其相關通路提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C type lectin related f ELISA Kit; C type lectin superfamily 2; member D ELISA Kit; C-type lectin domain family 2 member D ELISA Kit; CLAX ELISA Kit; CLC2D_HUMAN ELISA Kit; CLEC2D ELISA Kit; CLEC2D protein ELISA Kit; Clec2d5 ELISA Kit; L LT1 ELISA Kit; Lectin like NK cell receptor ELISA Kit; Lectin like transcript 1 ELISA Kit; Lectin-like NK cell receptor ELISA Kit; Lectin-like transcript 1 ELISA Kit; LLT-1 ELISA Kit; LLT1 ELISA Kit; OCIL ELISA Kit; Osteoclast inhibitory lectin ELISA Kit
縮寫：

CLEC2D
Uniprot No.：

Q9UHP7
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	95
	Range %	92-99
1:4	Average %	87
	Range %	83-91
1:8	Average %	90
	Range %	84-97

回收率：

The recovery of human CLEC2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	96	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.575	2.600	2.588	2.445
1000	2.215	2.147	2.181	2.038
500	1.533	1.498	1.516	1.373
250	0.901	0.944	0.923	0.780
125	0.531	0.565	0.548	0.405
62.5	0.365	0.344	0.355	0.212
31.25	0.258	0.250	0.254	0.111
0	0.139	0.147	0.143

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

CLEC-2 and zonulin are associated with MCI and AD Xin Wang, et al,Acta Neurologica Scandinavica,2019

功能：

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.
基因功能參考文獻：
1. These results show that susceptibility of normal articular chondrocytes to lysis by NK cells is modulated by NKR-P1A/LLT1 interactions. Thus, NKR-P1A/LLT1 interaction might provide some novel target for therapeutic interventions in the course of pathological cartilage injury. PMID: 29212911
2. Blocking LLT1-NKRP1A interaction will make prostate cancer cells susceptible to killing by NK cells, suggesting a therapeutic option for treatment of prostate cancer. PMID: 27626681
3. these data suggest that LLT1-CD161 interactions play a novel and important role in B cell maturation within the Germinal center in humans. PMID: 26829983
4. In RA joints, LLT1 is expressed by cells of the monocyte/macrophage lineage. PMID: 26147876
5. The hexamer of glycosylated LLT1 consists of three classical dimers. The hexameric packing may indicate a possible mode of interaction of C-type lectin-like proteins in the glycosylated form. PMID: 25760607
6. One polymorphism in LLT1 was found to be associated with our Crohn's Disease population (P<0.034).Our Ulcerative Colitis cohort was not associated with the variation in LLT1 (P=0.33) PMID: 22664939
7. LLT1 and CD161 have roles in modulating immune responses to pathogens; and interferon-gamma contributes to modulate immune responses PMID: 21930700
8. Molecular basis for LLT1 protein recognition by human CD161 protein (NKRP1A/KLRB1). PMID: 21572041
9. Data show that only CLEC2D isoform 1 (LLT1) is expressed on the cell surface. PMID: 20843815
10. LLT1 used Src-PTK, p38 and ERK signalling pathways, but not PKC, PI3K or calcineurin pathways, to increase production of IFN-gamma by human natural killer cells. PMID: 20415786
11. LLT1 induces Interferon Type II production by natural killer cells. PMID: 15104121
12. Data show that osteoclast inhibitory lectin (OCIL) binds a range of physiologically important glycosaminoglycans, and this property may modulate OCIL actions upon other cells [OCIL]. PMID: 15123656
13. Engagement of CD161 on NK cells with LLT1 expressed on target cells inhibited NK cell-mediated cytotoxicity and IFN-gamma secretion. LLT1/CD161 interaction in the presence of a TCR signal enhanced IFN-gamma production by T cells PMID: 16339512
14. LLT1 on target cells can inhibit NK cytotoxicity via interactions with CD161. LLT1 activates NFAT-GFP reporter cells expressing a CD3zeta-CD161 chimeric receptor; reciprocally, reporter cells with a CD3zeta-LLT1 chimeric receptor are stimulated by CD161 PMID: 16339513
15. Expression of LLT1 on activated dendritic cells and B cells suggests that it might regulate the cross-talk between NK cells and APCs PMID: 18453569
16. Women with a lysine (GG genotype) at position 19 of the OCIL protein displayed lower bone mineral density at femoral neck and at lumbar spine sites than women having an asparagine residue. PMID: 18465072
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亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Isoform 2]: Endoplasmic reticulum.; [Isoform 4]: Endoplasmic reticulum.
組織特異性：

Detected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1, isoform 2 and isoform 4 are expressed in T- and B-lymphocytes, and at lower levels in NK cells. They are also expressed in B-cell lines and LPS-matured monocyt
數據庫鏈接：

HGNC: 14351

OMIM: 605659

KEGG: hsa:29121

UniGene: Hs.268326

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.