Human Bone morphogenetic protein 3B(GDF10) ELISA kit

說明書下載

中文名稱：

人骨形態發生蛋白3B(GDF10)酶聯免疫試劑盒
貨號：

CSB-EL009343HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人骨形態發生蛋白3B(GDF10)酶聯免疫試劑盒（CSB-EL009343HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GDF10含量。GDF10（生長分化因子10）是一種TGF-β超家族成員，參與多種生物學過程，如骨骼形態發生、神經修復和腫瘤抑制。其作用機制涉及與TGF-β受體結合，激活下游信號通路，調節基因表達。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，該產品可廣泛應用于骨骼發育機制研究、纖維化疾病模型分析、藥物干預效果評價等科研領域，為探索GDF10在組織再生及病理調控中的分子機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

BIP ELISA Kit; BMP 3b ELISA Kit; BMP-3b ELISA Kit; BMP3B ELISA Kit; BMP3B_HUMAN ELISA Kit; Bone inducing protein ELISA Kit; Bone morphogenetic protein 3b [Precursor] ELISA Kit; Bone morphogenetic protein 3b ELISA Kit; Bone-inducing protein ELISA Kit; GDF 10 ELISA Kit; GDF-10 ELISA Kit; Gdf10 ELISA Kit; growth differentiation factor 10 ELISA Kit; Growth/differentiation factor 10 ELISA Kit
縮寫：

GDF10
Uniprot No.：

P55107
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GDF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	104-110
1:2	Average %	103
	Range %	101-105
1:4	Average %	88
	Range %	84-94
1:8	Average %	94
	Range %	91-97

回收率：

The recovery of human GDF10 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-103
EDTA plasma (n=4)	87	85-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.956	2.121	2.039	1.906
750	1.531	1.547	1.539	1.406
375	1.101	1.112	1.107	0.974
187.5	0.670	0.715	0.693	0.560
94	0.424	0.457	0.441	0.308
47	0.301	0.322	0.312	0.179
23.5	0.221	0.235	0.228	0.095
0	0.130	0.136	0.133

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis.
基因功能參考文獻：
1. Study shows that GDF10 is down-regulated in patients with oral squamous cell carcinoma, and is an independent risk factor for overall survival. Its expression is regulated by TGFBR3 which shares the signaling inhibiting epithelial-mesenchymal transition. PMID: 25728212
2. GDF10 is a stroke-induced signal for axonal sprouting and functional recovery. PMID: 26502261
3. new hypoxia-inducible and SOX9-regulated genes, Gdf10 and Chm-I. In addition, Mig6 and InhbA were induced by hypoxia, predominantly via HIF-2alpha PMID: 18077449
4. BMP3b and BMP6 genes were suppressed by DNA methylation and methylation of BMP3b is significantly frequent in Japanese malignant pleural mesotheliomas (MPMs), suggesting its pathogenic role and the ethnic difference in MPMs. PMID: 18949431
亞細胞定位：

Secreted.
蛋白家族：

TGF-beta family
組織特異性：

Expressed in femur, brain, lung, skeletal muscle, pancreas and testis.
數據庫鏈接：

HGNC: 4215

OMIM: 601361

KEGG: hsa:2662

STRING: 9606.ENSP00000224605

UniGene: Hs.2171

Human Bone morphogenetic protein 3B(GDF10) ELISA kit

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