Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人血管緊張素Ⅱ受體2抗體(AT2R-Ab)酶聯(lián)免疫試劑盒（CSB-E11255h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的AT2R Ab含量。試劑盒檢測范圍為0.312 U/mL-20 U/mL，適用于科研領(lǐng)域探索AT2R在生理病理過程中的作用機(jī)制；其抗體水平變化可能與心血管疾病、纖維化疾病等研究相關(guān)；適用于基礎(chǔ)研究中評估疾病模型動物的抗體動態(tài)變化、體外藥物干預(yù)實驗的效應(yīng)分析，或篩選調(diào)控AT2R信號通路的潛在化合物；為心血管、腎臟等系統(tǒng)相關(guān)分子機(jī)制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

angiotensin 2 receptor 2 antibody, AT2R-Ab
縮寫：

AT2R Ab
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.312 U/mL-20 U/mL
靈敏度：

0.078 U/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AT2R-Ab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	82-105
1:2	Average %	94
	Range %	84-100
1:4	Average %	98
	Range %	92-101
1:8	Average %	94
	Range %	82-105

回收率：

The recovery of human AT2R-Ab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-105
EDTA plasma (n=4)	95	85-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.