Human Acyloxyacyl hydrolase(AOAH) ELISA kit

說明書下載

中文名稱：

人酰基羧酸水解酶AOAH）酶聯(lián)免疫試劑盒
貨號：

CSB-EL001853HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人酰基羧酸水解酶AOAH）酶聯(lián)免疫試劑盒（CSB-EL001853HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的AOAH含量。AOAH（酰基羧酸水解酶）是一種在多種細胞中表達的宿主脂肪酶，能夠選擇性地去除革蘭氏陰性菌LPS脂質(zhì)A的二級酰基鏈，從而滅活LPS，減輕炎癥和組織損傷。研究表明，AOAH在感染性疾病中發(fā)揮重要作用，同時也在過敏性哮喘、銀屑病等自身免疫性疾病中扮演重要角色。研究機制顯示，AOAH通過降解LPS，調(diào)節(jié)免疫反應(yīng)，減輕炎癥。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，其應(yīng)用場景包括但不限于脂質(zhì)代謝異常研究、炎癥信號通路解析、免疫細胞功能評估等基礎(chǔ)科研領(lǐng)域，例如在動脈粥樣硬化模型、肝臟疾病或腸道菌群相關(guān)免疫調(diào)控實驗中定量追蹤AOHA的表達動態(tài)變化，為揭示其在病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Acyloxyacyl hydrolase (neutrophil) ELISA Kit; Acyloxyacyl hydrolase large subunit ELISA Kit; AOAH ELISA Kit; AOAH_HUMAN ELISA Kit
縮寫：

AOAH
Uniprot No.：

P28039
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AOAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	99
	Range %	82-106
1:8	Average %	87
	Range %	81-92

回收率：

The recovery of human AOAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	101	94-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.680	2.769	2.725	2.620
5	2.246	2.124	2.185	2.080
2.5	1.495	1.387	1.441	1.336
1.25	0.820	0.837	0.829	0.724
0.625	0.475	0.486	0.481	0.376
0.312	0.281	0.272	0.277	0.172
0.156	0.204	0.210	0.207	0.102
0	0.104	0.105	0.105

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.
基因功能參考文獻：
1. analysis of AOAH function through its crystal structure and complex with lipopolysaccharide PMID: 29343645
2. AOAH - Gene involved in innate immunity that have been associated with Chronic Rhinosinusitis. PMID: 27888910
3. AOAH rs60023210 variants may be associated with carotid intima-media thickness at bifurcation. PMID: 26319989
4. Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975
亞細胞定位：

Secreted. Cytoplasmic vesicle.
數(shù)據(jù)庫鏈接：

HGNC: 548

OMIM: 102593

KEGG: hsa:313

STRING: 9606.ENSP00000258749

UniGene: Hs.488007

Human Acyloxyacyl hydrolase(AOAH) ELISA kit

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