Horse platelet-derived growth factor-BB(PDGF-BB) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

馬血小板衍生生長因子BB(PDGF-BB)酶聯免疫試劑盒（CSB-EQ027241HO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿樣本中的PDGF-BB含量。PDGF - BB即血小板衍生生長因子 - BB，在細胞生長、增殖、遷移及血管生成等生理過程中發揮重要作用。其異常表達與腫瘤、心血管疾病等多種病理狀況相關。研究聚焦于其信號通路機制，期望通過干預該靶點開發新的治療藥物和策略。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研場景中探究心血管疾病模型、創傷修復機制或干細胞分化調控等相關課題，為體外實驗中PDGF-BB的功能研究提供可靠的數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PDGF-BB
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma,tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	91-99
1:4	Average %	84
	Range %	81-87
1:8	Average %	83
	Range %	80-86

回收率：

The recovery of horse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-109
EDTA plasma (n=4)	94	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.