Goat gonadotropin-releasing hormone,GnRH ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

山羊促性腺激素釋放激素(GnRH)酶聯(lián)免疫試劑盒（CSB-E13276G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GnRH含量。GnRH（促性腺激素釋放激素）是下丘腦分泌的十肽激素，在生殖調(diào)節(jié)中起關(guān)鍵作用。其作用機(jī)制為脈沖式分泌，刺激垂體前葉合成和釋放促性腺激素，進(jìn)而調(diào)控性腺功能。對GnRH的研究有助于了解生殖生理及攻克相關(guān)疾病。試劑盒檢測范圍為2.5 pg/mL-500 pg/mL，該產(chǎn)品可廣泛應(yīng)用于山羊等反芻動物的生殖周期研究、繁殖調(diào)控機(jī)制探索、激素水平動態(tài)監(jiān)測以及環(huán)境內(nèi)分泌干擾物評估等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GnRH
Uniprot No.：

D3YJ02
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.5 pg/mL-500 pg/mL
靈敏度：

1.25 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	95
	Range %	88-102
1:4	Average %	94
	Range %	89-99
1:8	Average %	95
	Range %	89-103

回收率：

The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-102
EDTA plasma (n=4)	97	91-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.