Goat Tumor necrosis factor α,TNF-α ELISA KIT

產(chǎn)品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

山羊腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E09811g）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。TNF（腫瘤壞死因子）是一種具有多種生物活性的細胞因子。它參與免疫調(diào)節(jié)、炎癥反應(yīng)等生理過程。其研究機制包括與受體結(jié)合激活信號通路，調(diào)節(jié)基因表達。異常的 TNF 表達與多種疾病如類風(fēng)濕關(guān)節(jié)炎等相關(guān)，也是藥物研發(fā)的重要靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，支持科研人員開展山羊炎癥模型建立、免疫機制研究、疫苗效力評估等實驗，為畜牧獸醫(yī)研究、動物疾病模型開發(fā)及生物制品質(zhì)量控制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
縮寫：

TNF
Uniprot No.：

P13296
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-91
1:2	Average %	98
	Range %	91-103
1:4	Average %	99
	Range %	95-104
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of goat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	97	92-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻：
1. Data indicate that three single-nucleotide polymorphisms were identified in tumor necrosis factor-alpha (TNF-alpha) gene. PMID: 25966128
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數(shù)據(jù)庫鏈接：

KEGG: chx:100861232

UniGene: Chi.11711