• A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
• Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
• HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
• Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
• Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
• Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
• Substrate A (1x 7 ml) --Mix with Sbustrate B.
• Substrate B (1x 7 ml) --Mix with Sbustrate A
• Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells)
• An Instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water

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