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中文名稱：魚多巴胺(DA)酶聯(lián)免疫試劑盒
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貨號：CSB-EQ027496FI
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規(guī)格：96T/48T
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價格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：魚多巴胺(DA)酶聯(lián)免疫試劑盒（CSB-EQ027496FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DA含量。DA即多巴胺，是腦內(nèi)重要神經(jīng)遞質(zhì)，在運動控制、認知、情感及內(nèi)分泌調(diào)節(jié)等過程中發(fā)揮關(guān)鍵作用。研究機制方面，圍繞其合成、釋放、與受體結(jié)合及代謝等環(huán)節(jié)展開，探究其在帕金森病、精神分裂癥等疾病中異常變化以尋找治療靶點。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，該產(chǎn)品適用于魚類神經(jīng)生物學(xué)研究、水產(chǎn)養(yǎng)殖健康監(jiān)測、環(huán)境污染物對水生生物神經(jīng)系統(tǒng)的毒性評估等科研領(lǐng)域，特別在水體環(huán)境毒理學(xué)實驗中，可通過檢測魚類血液或組織DA水平評估重金屬、農(nóng)藥等污染物對中樞神經(jīng)系統(tǒng)的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：DA
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種屬：Fish
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：62.5 pg/mL-1000 pg/mL
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靈敏度：62.5 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Neuroscience
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 92-104 1:2 Average % 94 Range % 91-98 1:4 Average % 102 Range % 98-106 1:8 Average % 88 Range % 82-94 -
回收率：
The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 103 98-107 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1000 0.154 0.155 0.155 500 0.278 0.272 0.275 250 0.503 0.518 0.511 125 1.135 1.174 1.155 62.5 2.345 2.428 2.387 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- Integrated Analysis of Neuroendocrine and Neurotransmission Pathways Following Developmental Atrazine Exposure in Zebrafish SC Stradtman, JN Swihart, K Moore, IN Akoro,International Journal of Molecular Sciences,2024
- Neuroprotective effects of nanogold-based Ayurveda medicine Suvarna Bhasma against rotenone-induced Parkinson's-like model S Biswas,Journal of Ayurveda and integrative medicine,2023
- Differential Developmental Neurotoxicity and Tissue Uptake of the Per-and Polyfluoroalkyl Substance Alternatives, GenX and PFBS O Wasel,Environmental science & technology,2023
- Effects of two alternative plasticizers on the growth hormone-related endocrine system, neurodevelopment, and oxidative stress of zebrafish larvae K Yun,Environmental pollution,2023
- Low concentrations of the antidepressant venlafaxine affect courtship behaviour and alter serotonin and dopamine systems in zebrafish (Danio rerio) Y Tang,Aquatic toxicology,2023
- Potential Adverse Outcome Pathway of Oxidative Stress Induction and Neurodevelopmental Toxicity Mediated by Three Alkyl Organophosphate Flame Retardants in Zebrafish Larvae CH Lee,/,2024
- Different action mechanisms of low-and high-level quercetin in the brains of adult zebrafish (Danio rerio) X Wu,Ecotoxicology and Environmental Safety,2021
- Assessment of the Effects of Bisphenol A on Dopamine Synthesis and Blood Vessels in the Goldfish Brain Wang Q, et al,International Journal of Molecular Sciences,2019
- Venlafaxine plus melatonin ameliorate reserpine-induced depression-like behavior in zebrafish Ya-Qiu Tang, et al,Neurotoxicology and Teratology,2019
- Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus Su M,BMC Genomics,2019
- Dopamine regulates renal osmoregulation during hyposaline stress via DRD1 in the spotted scat (Scatophagus argus) Maoliang Su.et al,Scientific Reports,2016
- Changes of physiological rhythms of N-methyl-d-aspartate receptors in the chum salmon Oncorhynchus keta: effect of seawater acclimation during the parr-smolt transformation Young Jae Choi.et al,Biological Rhythm Research,2016
- Ambient particulate air pollution and circulating antioxidant enzymes: A repeated-measure study in healthy adults in Beijing, China Shaowei Wu. et al,Environ Pollut,2015
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