Fish Insulin-like growth factor 1, IGF-1 ELISA Kit
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中文名稱：魚(yú)胰島素樣生長(zhǎng)因子1(IGF-1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E12122Fh
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：魚(yú)胰島素樣生長(zhǎng)因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E12122Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IGF-1含量。IGF-1即胰島素樣生長(zhǎng)因子-1，它在細(xì)胞的生長(zhǎng)、分化和增殖等生理過(guò)程中發(fā)揮重要作用。其研究機(jī)制涉及與相應(yīng)受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞的存活與功能。目前在生長(zhǎng)發(fā)育、代謝等多方面研究廣泛，也與某些疾病的發(fā)生發(fā)展相關(guān)。試劑盒檢測(cè)范圍為25 pg/mL-2000 pg/mL，可為水產(chǎn)動(dòng)物生理研究、養(yǎng)殖管理優(yōu)化以及環(huán)境脅迫響應(yīng)機(jī)制探索提供可靠工具，特別適用于實(shí)驗(yàn)室條件下魚(yú)類生長(zhǎng)性能評(píng)估、飼料效應(yīng)分析以及激素調(diào)控網(wǎng)絡(luò)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
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縮寫(xiě)：IGF-1
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Uniprot No.：
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種屬：Fish
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：25 pg/mL-2000 pg/mL
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靈敏度：31.25 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 90-98 1:2 Average % 95 Range % 91-99 1:4 Average % 102 Range % 98-106 1:8 Average % 87 Range % 82-93 -
回收率：
The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-99 EDTA plasma (n=4) 88 82-94 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 2000 0.341 0.343 0.342 750 0.476 0.484 0.480 375 0.682 0.636 0.659 125 1.067 1.072 1.070 25 1.488 1.567 1.528 0 1.955 1.995 1.975 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Integrated aquaculture of whiteleg shrimp Litopenaeus vannamei and European seabass Dicentrarchus labrax: impacts on performance, welfare, blood physiological response, carcass traits, productivity, and farm profitability AIG Elhetawy, AT Mansour, AM Lotfy,Aquaculture International,2025
- Dietary grape seed extract mitigated growth retardation, hormonal delay, and gastrointestinal toxicity induced by insecticide imidacloprid in Nile tilapia NA El-Fahla, H Abdelrazek, AS Fouad,Fish Physiology and Biochemistry,2025
- Dietary rosemary oil with/without zymogen forte improves water quality, growth hormones, immune-physiological response, stress resilience, and health status of Chelon ramada grown in groundwater AIG Elhetawy, MF El Basuini, AIA Mansour,BMC Veterinary Research,2025
- Analysis of egg quality and growth-related factors during embryogenic development of olive flounder (Paralichthys olivaceus) fed different broodstock diets JS Moon, M Seong, SC Kim, J Cadangin,Fisheries and Aquatic Sciences,2024
- Farkl? süre ve dozlarda gümü? nanopartikül (AgNP) uygulanan sazan bal?klar?nda kan serumu BH ve IGF-1 düzeylerine etkisi D Ay Güng?r,Thesis&Dissertation,2024
- Thyroid and growth hormone endocrine disruption and mechanisms of homosalate and octisalate using wild-type, thrαa-/-, and dre-miR-499-/- zebrafish embryo/larvae Y Ka, I Lee, K Ji,Ecotoxicology and Environmental Safety,2024
- Massive stocking of chum salmon (Oncorhynchus keta) fry fattens non-native brown trout (Salmo trutta) in Hokkaido, Japan K Honda,PloS one,2024
- Progressive substitution of fish oil with Schizochytrium-derived algal oil in the diet of Atlantic salmon (Salmo salar) parr subjected to winter signal period NW Farris,Aquaculture Reports,2024
- Effects of dietary supplementation of Bacillus, β-glucooligosaccharide and their synbiotic on the growth, digestion, immunity, and gut microbiota profile of abalone, Haliotis discus hannai J Cadangin,Aquaculture Reports,2024
- Tissue explants as tools for studying the epigenetic modulation of the GH-IGF-I axis in farmed fish E Perera,Frontiers in physiology,2024
- Raw Fish-based Moist Pellet and Extruded Pellet on Growth, Levels and Expression of GH/IGF Axis, Intestinal Enzyme Activity and Gastrointestinal Morphology of … CY Jeon,/,2023
- Effects on Growth Hormone, Insulin-Like Growth Factors, and Oxidative Stress in Zebrafish Larvae after Exposure to Acetyl Tributyl Citrate and Acetyl Triethyl Citrate K Yun,Environmental pollution,2023
- Combined Effects of Preservative Chemicals in Consumer Products Using Embryonic and Adult Zebrafish C Park,/,2023
- Effect of water temperature on walleye pollock (Gadus chalcogrammus) embryos, larvae and juveniles: Survival, HSP70 expression, and physiological responses SS Kim,Aquaculture,2023
- Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata) A Amri,Aquaculture,2023
- Effects of Stocking Density on Stress, Hematological Responses, and Growth of Black Rockfish Sebastes schlegelii JW Lee,Journal of aquatic animal health,2023
- The Combined Effects of Preservative Chemicals in Consumer Products: An Analysis Using Embryonic and Adult Zebrafish T Wang,/,2024
- Fibrinogen beta chain may be a potential predict biomarker for pre-eclampsia: a preliminary study J Shi,Animals,2022
- Low extrusion pressure and small feed particle size improve the growth performance and digestive physiology of rockfish (Sebastes schlegeli) JH Cho,Aquaculture,2022
- Schizochytrium-derived algal oil as replacement for fish oil-effect on growth and smoltification process of Atlantic salmon (Salmo salar L.) DH Blaauw,Nord universitet,2022
- Effects of black soldier fly (Hermetia illucens) pre-pupae meal on the growth, stress, and immune responses of juvenile rainbow trout (Oncorhynchus mykiss) reared at different stocking densities JH Cho,Aquaculture Reports,2022
- Determination of acute toxicity of sodium pyrithione on common carp and its effects on some hormones and hematological parameters N Korkmaz,Research Square,2022
- Differential effects of two seawater transfer regimes on the hypoosmoregulatory adaptation, hormonal response, feed efficiency, and growth performance of juvenile steelhead trout M Lee,Aquaculture Reports,2022
- A preliminary study of dietary protein requirement of juvenile marbled flounder (Pseudopleuronectes yokohamae) JH Cho,Animal Nutrition,2021
- The IGF-1/GH-GLUTs-plasma glucose regulating axis in hybrid grouper (Epinephelus fuscoguttatus♀ × epinephelus lanceolatus♂) fed a high-carbohydrate diet Xiaoyi Wu,General And Comparative Endocrinology,2021
- The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
- Effect of melatonin and folic acid supplementation on the growth performance, antioxidant status, and liver histology of the farmed gilthead sea bream (Sparus aurata L.) under standard rearing conditions A Amri,Fish Physiology and Biochemistry,2020
- Environmental Hypoxia Causes Growth Retardation, Osteoclast Differentiation and Calcium Dyshomeostasis in Juvenile Rainbow Trout (Oncorhynchus mykiss) Zhi-Shuai Hou, et al,Science of The Total Environment,2019
- A temperature shift on the migratory route similarly impairs hypo-osmoregulatory capacities in two strains of Atlantic salmon (Salmo salar L.) smolts Bernard B,Fish Physiology and Biochemistry,2019
- THE EFFECTS OF PESTICIDE NEEMAZAL ON THE GROWTH HORMONE (GH) AND INSULIN-LIKE GROWTH HORMONE (IGF-1) OF CARP FISH (Cyprinus Tihomir Dovramadjiev.et al,researchgate,2016
- Growth response, body composition, plasma metabolites, digestive and antioxidant enzymes activities of Siberian sturgeon (Acipenser baerii, Brandt, 1869) fed different dietary protein and carbohydrate: lipid ratio Babaei S.et al,Aquaculture Research,2016
- Enteric, hepatic and muscle tissue development of juvenile dourado Salminus brasiliensis fed with lyophilized bovine colostrum Wiolene Montanari Nordi. et al, Fisheries Science,2016
- Cellular activity and development of enteric, hepatic and muscle tissues of juvenile pacu Piaractus mesopotamicus (Holmberg 1887) fed with lyophilized bovine colostrum Wiolene Montanari Nordi. et al,Aquaculture Research ,2015
- Effects of recombinant growth hormone on growth factor and immune component levels in the cinnamon clownfish, Amphiprion melanopus Na Na Kim et al,Marine Biology Research,2014
- Molecular cloning, expression analysis of insulin-like growth factor I (IGF-I) gene and IGF-I serum concentration in female and male Tongue sole (Cynoglossus semilaevis) /,Brain Research,2011
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相關(guān)問(wèn)答
■ 常見(jiàn)問(wèn)題解答
Q:
I want to use Fish Igf-1 ELISA kit for fish's liver homogenate.
In the protocol of the kit, for tissue homogenates, after homogenizing, stored overnight at -20oC. After 2 freeze-thaw cycles, centrifuge the homogenates.I do not understand too much about this. It means I have wait for 2 days to homogenate 2 sample (homogenate-> store -20oC-> thaw-> store 20oC -> thaw-> centrifuge)? Can you explain for me. Thanks!
A:
Thanks for your inquiry.Regarding tissue sample,pls store the sample which has been treated by PBS at -20 degrees overnight.After that,take the sample at room temperature and let it be thawed freely (the first freeze thaw cycle).Then freeze it at -80 degrees or store it at -20 degrees overnight.Then take it at temperature and let it be thawed freely.This is the 2nd freeze-thaw cycle.Regarding the later procedures,pls follow the manual.
If any further questions,pls let me know.
If any further questions,pls let me know.
Q:
Can you let me know in which buffer the samples should be diluted?
This is not written in de manual!
A:
Thanks for your inquiry.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.
Q:
In the current protocol nothing is mentioned about an extraction of IGF-1 from binding proteins. This is generally a necessary procedure to properly measure IGF-1. Is an extraction, and if so which, recommended by Cusabio? Is this procedure in the most recent protocol?
A:
Thanks for your inquiry. There is no need to do extraction of IGF-1 from binding proteins for this kit.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.
靶點(diǎn)詳情
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功能：The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
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基因功能參考文獻(xiàn)：
- Nutrient availability, IGF-I, and genetic variation affect weight loss, in part through alterations of proteolytic pathways in rainbow trout. PMID: 19726716
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亞細(xì)胞定位：Secreted.
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蛋白家族：Insulin family
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數(shù)據(jù)庫(kù)鏈接：
UniGene: Omy.7104
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