Dog β2-microglobulin,BMG/β2-MG ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

犬β2微球蛋白(BMG/β2-MG)酶聯(lián)免疫試劑盒（CSB-E13768c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的B2M含量。B2M全稱為β2微球蛋白，其在免疫識別、抗原呈遞等過程中發(fā)揮重要作用。它與主要組織相容性復合體Ⅰ類分子結(jié)合，參與機體免疫監(jiān)視。研究發(fā)現(xiàn)其表達異常與腫瘤、自身免疫病等多種疾病相關(guān)，目前研究聚焦于其在疾病發(fā)生發(fā)展機制及作為治療靶點的潛力。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，適用于科研場景下犬類腎臟功能評估、免疫相關(guān)疾病機制研究或藥物毒性實驗中生物標志物的檢測，為動物實驗提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

B2M ELISA Kit; Beta-2-microglobulin ELISA Kit; Fragment ELISA Kit
縮寫：

B2M
Uniprot No.：

P19341
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 μg/mL-50 μg/mL
靈敏度：

0.2 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	92
	Range %	85-100
1:4	Average %	95
	Range %	85-105
1:8	Average %	95
	Range %	88-102

回收率：

The recovery of dog β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-99
EDTA plasma (n=4)	95	91-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

UniGene: Cfa.29179