Dog growth hormone,GH ELISA Kit

Dog growth hormone,GH ELISA Kit

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文獻引用 (1) 說明書下載

中文名稱：

犬生長激素(GH)酶聯免疫試劑盒
貨號：

CSB-E14962c
規格：

96T/48T
價格：

￥3900/￥2500
促銷：
其他：

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產品描述：

犬生長激素(GH)酶聯免疫試劑盒（CSB-E14962c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 即生長激素 1 基因，它編碼生長激素，在人體生長發育、代謝等生理過程中扮演重要角色。研究機制方面，主要圍繞其調控生長激素合成與分泌、與生長信號通路的相互作用等，這些研究有助于深入理解生長發育異常機制及相關疾病治療。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產品適用于動物生長機制研究、代謝調控分析、藥物或營養干預實驗等科研領域，為探究犬類生理病理過程中的GH動態變化提供穩定工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
縮寫：

GH1
Uniprot No.：

P33711
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	98
	Range %	91-105
1:4	Average %	89
	Range %	84-95
1:8	Average %	99
	Range %	92-106

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.853

2.734

2.794

2.632

2.087

2.067

2.077

1.916

1.504

1.498

1.501

1.339

0.897

0.929

0.913

0.751

2.5

0.550

0.521

0.536

0.374

1.25

0.383

0.363

0.373

0.211

0.625

0.271

0.284

0.277

0.116

0.158

0.165

0.162

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Age-specific variations in hematological and biochemical parameters in middle-and large-sized of dogs SH Lee,J Vet Sci,2019

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能參考文獻：
1. Progesterone alone has no direct stimulatory effect on GH transcription in mammary gland. PMID: 18262383
2. Study provides evidence that progesteron might increase autocrine Growth Hormone production which might directly stimulate local or systemic IGF-I secretion in canine mammary tumours. PMID: 18367392
亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: cfa:403795

STRING: 9615.ENSCAFP00000018659

UniGene: Cfa.3656

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.