Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒（CSB-E13345c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BGLAP含量。BGLAP即骨鈣素，是骨組織中最為豐富的非膠原蛋白。其背景上，與骨骼生長、代謝密切相關。研究機制方面，它可調節骨代謝平衡，還在能量代謝、生殖等過程中發揮作用，目前圍繞它在疾病診治中的潛在價值是研究熱點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研人員可將其應用于犬類骨骼發育研究、骨質疏松模型構建、骨代謝相關藥物篩選，以及營養干預對骨組織影響等基礎研究領域，為犬科動物骨生物學研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
縮寫：

BGLAP
Uniprot No.：

P81455
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	87-102
1:2	Average %	92
	Range %	89-98
1:4	Average %	89
	Range %	84-96
1:8	Average %	90
	Range %	86-99

回收率：

The recovery of dog OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-96
EDTA plasma (n=4)	102	95-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
亞細胞定位：

Secreted.
蛋白家族：

Osteocalcin/matrix Gla protein family
數據庫鏈接：

STRING: 9615.ENSCAFP00000024773

UniGene: Cfa.3627