Dog Interleukin 18,IL-18 ELISA KIT

Dog Interleukin 18,IL-18 ELISA KIT

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中文名稱：

犬白介素18(IL-18)酶聯免疫試劑盒
貨號：

CSB-E09848c
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬白介素18(IL-18)酶聯免疫試劑盒（CSB-E09848c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL18含量。IL18是一種細胞因子，屬于白細胞介素家族。它在免疫反應中發揮重要作用，能調節Th1、NK等細胞的活性。研究機制上，IL18可通過與受體結合激活下游信號通路，參與炎癥反應和免疫調節，其異常表達與多種疾病相關，是藥物研發的潛在靶點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研領域對犬類炎癥性疾病（如敗血癥、自身免疫病）、腫瘤免疫微環境或疫苗免疫效果的研究，支持多類型樣本同步檢測，為動物實驗中的免疫指標分析提供標準化工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
縮寫：

IL18
Uniprot No.：

Q9XSR0
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-109
1:2	Average %	97
	Range %	91-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.675	2.552	2.614	2.439
400	2.065	2.076	2.071	1.896
200	1.372	1.424	1.398	1.223
100	0.726	0.739	0.733	0.558
50	0.471	0.504	0.488	0.313
25	0.380	0.359	0.370	0.195
12.5	0.271	0.259	0.265	0.090
0	0.173	0.177	0.175

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
亞細胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

KEGG: cfa:403796

STRING: 9615.ENSCAFP00000038185

UniGene: Cfa.40

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.