Dog Apolipoprotein A-I(APOA1) ELISA kit

Dog Apolipoprotein A-I(APOA1) ELISA kit

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中文名稱：

犬載脂蛋白A1(Apo-A1)酶聯免疫試劑盒
貨號：

CSB-EL001913DO
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬載脂蛋白A1(Apo-A1)酶聯免疫試劑盒（CSB-EL001913DO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是一種位于細胞膜外的分泌蛋白，是高密度脂蛋白（HDL）的主要結構載脂蛋白，在逆向膽固醇轉運途徑中起關鍵作用。其研究機制涉及膽固醇代謝、抗炎、抗動脈粥樣硬化等功能，是心血管疾病預防和治療中的潛在靶點。試劑盒檢測范圍為28.12 ng/mL-1800 ng/mL，該產品適用于科研領域中對犬類脂代謝機制、心血管疾病模型構建、營養干預效果評估等研究，尤其在探究HDL功能與疾病相關性、藥物或膳食成分對脂代謝影響的實驗中具有廣泛應用。組織勻漿兼容性設計還可支持特定器官或病理組織中Apo - A1的分布研究，為深入探索犬類脂質代謝的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
縮寫：

APOA1
Uniprot No.：

P02648
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

28.12 ng/mL-1800 ng/mL
靈敏度：

7 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	93
	Range %	89-103
1:2000	Average %	89
	Range %	85-95
1:4000	Average %	95
	Range %	84-103
1:8000	Average %	93
	Range %	83-98

回收率：

The recovery of dog APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	92-95
EDTA plasma (n=4)	95	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1800	0.142	0.145	0.144
900	0.215	0.229	0.222
450	0.382	0.367	0.375
225	0.551	0.558	0.555
112.5	0.973	0.990	0.982
56.25	1.697	1.623	1.660
28.12	2.287	2.328	2.308
0	2.654	2.761	2.708

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Oral nicotinic acid administration effect on lipids, thyroid hormones, and oxidative stress in intact adult dogs N Derakhshandeh, S Nazifi, A Mogheiseh,BMC Veterinary Research,2025

功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
亞細胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
組織特異性：

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
數據庫鏈接：

STRING: 9615.ENSCAFP00000019630

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.