Chicken Interferon γ,IFN-γ ELISA Kit

Chicken Interferon γ,IFN-γ ELISA Kit

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中文名稱：

雞γ干擾素(IFN-γ)酶聯免疫試劑盒
貨號：

CSB-E08550Ch
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

雞γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E08550Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。干擾素-γ（IFNG）是一種II型干擾素，主要由自然殺傷細胞和活化的T細胞產生，具有抗病毒、免疫調節和抗腫瘤特性。其通過結合干擾素伽瑪受體（IFNGR）調節JAK-STAT通路，激活抗原提呈細胞，促進I型輔助T細胞（Th1細胞）的分化，并抑制破骨細胞形成。研究機制涉及多種生物過程和細胞信號通路，如蛋白導入細胞核、轉錄調控、細胞凋亡和免疫反應等。試劑盒檢測范圍為3.125 pg/mL-800 pg/mL，適用于家禽免疫機制研究、疫苗效力評估以及感染性疾病模型中免疫應答動態監測，如禽流感、新城疫等病毒性疾病的免疫調控機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
縮寫：

IFNG
Uniprot No.：

P49708
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.125 pg/mL-800 pg/mL
靈敏度：

3.125 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-100
1:2	Average %	92
	Range %	88-97
1:4	Average %	102
	Range %	98-106
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
800	0.178	0.182	0.180
200	0.245	0.233	0.239
50	0.457	0.482	0.470
12.5	0.848	0.817	0.833
3.125	1.396	1.364	1.380
0	2.237	2.242	2.240

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The inhibitory effect of Hypericum japonicum on H9N2 avian influenza virus H Hu, J Li, S Zheng, K Zhao, Y Xia, X Wei, M Han,Advanced Biotechnology,2024
Investigation of the Potential of Heterophil/Lymphocyte Ratio as a Biomarker to Predict Colonization Resistance and Inflammatory Response to Salmonella enteritidis Infection in Chicken M Thiam,Pathogens,2023
Effect of Two Particle Sizes of Nano Zinc Oxide on Growth Performance, Immune Function, Digestive Tract Morphology, and Intestinal Microbiota Composition in Broilers J Qu,Animals,2023
Impact of stocking density and dietary nano-zinc supplementation on stress indicators, immunity, and DNA damage in broiler chickens ? Sevim,Journal of the Hellenic Veterinary Medical Society,2023
Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under Salmonella enterica Serovar Typhimurium Infection Z Wang,Microorganisms,2022
Synergistic Immunosuppression of Avian Leukosis Virus Subgroup J and Infectious Bursal Disease Virus Is Responsible for Enhanced Pathogenicity W Chen,Viruses,2022
Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,Parasitology Research,2021
Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,Animals,2019
Immunogenic potential and protective efficacy of a sptP deletion mutant of Salmonella Enteritidis as a live vaccine for chickens against a lethal challenge Yaxin Guo, et al,International Journal of Medical Microbiology,2019
Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union Metayer JP, et al,Toxins,2019
The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,2019
Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood N. Joshi, et al,Tropical animal science journal,2019
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,2019
Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,2018
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
Gene cloning, expression and immune adjuvant properties of the recombinant fusion peptide Ta1-BLP on avian influenza inactivate virus vaccine CongZhang.et al,Microbial Pathogenesis,2018
Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,Parasites & Vectors,2018
Validation of immunomodulatory effects of lipopolysaccharide through expression profiling of Th1 and Th2 biased genes in Newcastle disease virus vaccinated indigenous chicken Rabia Bhardwaj.et al,Veterinary World,2018
Influenza Inactive Virus Vaccine with the Fusion Peptide (rTα1- BP5) Enhances Protection Against Influenza Through Humoral and Cell-Mediated Immunity Chen Wang.et al,,2016
Heat stress decreases expression of the cytokines, avian β-defensins 4 and 6 and Toll-like receptor 2 in broiler chickens infected with Salmonella Enteritidis W.M. Quinteiro-Filho.et al,Veterinary Immunology and Immunopathology,2017
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,2016
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
Effect of Antibiotic Growth Promoters on Anti-oxidative and Anti-inflammatory Activities in Broiler Chickens Kabploy K.et al,The Thai Journal of Veterinary Medicine,2016
Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015
Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,Lipids in Health and Disease,2012
Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,Virology Journal,2011
Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,Tumor Biology,2010
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction Nirala BK. et al,Diab Vasc Dis Res,2015
Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek's disease virus Matsuyama-Kato A et al,Arch Virol,2014
Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV /,Comparative Immunology, Microbiology and Infectious Diseases,2011
Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens /,clinical and vaccine immunology,2011

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最新研究進展：

IFNG，全稱為γ-干擾素，是一種由免疫細胞產生的細胞因子，在免疫反應中起重要作用。最新研究發現，IFNG在免疫系統中調節T細胞、B細胞和自然殺傷細胞等免疫細胞的功能。IFNG還在感染和炎癥過程中發揮重要作用。研究表明，IFNG水平升高與多種疾病有關，包括自身免疫病、感染性疾病、腫瘤、心血管疾病和神經退行性疾病等。近年來，研究人員正努力探索利用IFNG的治療作用，例如用IFNG治療乳腺癌、肺癌和黑色素瘤等。
功能：

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
基因功能參考文獻：
1. Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID: 25673902
2. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID: 22113479
3. The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID: 17888472
4. The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID: 17899460
5. that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID: 19070879
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亞細胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
數據庫鏈接：

KEGG: gga:396054

STRING: 9031.ENSGALP00000016086

UniGene: Gga.916

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.