Canine follicle-stimulating hormone,FSH ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬促卵泡素(FSH)酶聯免疫試劑盒（CSB-E06868c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSH含量。FSH即促卵泡生成素，是腺垂體分泌的糖蛋白激素，對生殖系統發育和功能維持至關重要。其研究機制主要圍繞調節卵泡發育、精子生成等生殖過程，還涉及與下丘腦-垂體-性腺軸的相互作用，對不孕不育等疾病的治療有重要意義。試劑盒檢測范圍為0.35 mIU/mL-140 mIU/mL，科研人員可將其應用于犬類生殖生理學研究、繁殖障礙機制探索、生殖相關藥物或營養干預效果評估等領域，例如通過檢測發情周期、卵巢功能異?；虿G丸發育相關樣本中的FSH動態變化，為犬科動物繁殖生物學研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

FSH
Uniprot No.：

E2RJZ8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.35 mIU/mL-140 mIU/mL
靈敏度：

0.35 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	95
	Range %	90-105
1:4	Average %	98
	Range %	93-104
1:8	Average %	92
	Range %	85-99

回收率：

The recovery of canine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	96	92-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.