Canine Matrix metalloproteinase 1,MMP-1 ELISA kit

Canine Matrix metalloproteinase 1,MMP-1 ELISA kit

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中文名稱：

犬基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14057c
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒（CSB-E14057c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP-1含量。MMP-1，又稱間質(zhì)膠原酶和成纖維細(xì)胞膠原酶，是基質(zhì)金屬蛋白酶家族的一員，主要參與細(xì)胞外基質(zhì)的降解。其在正常生理過程中如胚胎發(fā)育和組織重塑中起作用，同時(shí)在疾病過程中如癌癥和骨關(guān)節(jié)炎中，MMP-1的表達(dá)和活性增加，導(dǎo)致細(xì)胞外基質(zhì)破壞，促進(jìn)疾病進(jìn)展。試劑盒檢測(cè)范圍為0.18 ng/mL-12 ng/mL，適用于科研場(chǎng)景中探究犬類疾病模型（如關(guān)節(jié)炎、皮膚損傷修復(fù)或腫瘤微環(huán)境）中MMP-1的動(dòng)態(tài)變化。可為分子機(jī)制研究、藥物開發(fā)或生物標(biāo)志物篩選提供定量數(shù)據(jù)支持，滿足科研工作者對(duì)犬類MMP-1活性監(jiān)測(cè)的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MMP-1
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.18 ng/mL-12 ng/mL
靈敏度：

0.04 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-102
1:2	Average %	89
	Range %	85-99
1:4	Average %	94
	Range %	85-97
1:8	Average %	90
	Range %	83-98

回收率：

The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	82-98
EDTA plasma (n=4)	97	89-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
12	2.309	2.326	2.318	2.114
6	1.754	1.699	1.727	1.523
3	1.301	1.338	1.320	1.116
1.5	0.931	0.920	0.926	0.722
0.75	0.573	0.585	0.579	0.375
0.37	0.412	0.407	0.410	0.206
0.18	0.299	0.303	0.301	0.097
0	0.201	0.206	0.204

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.