Canine Matrix metalloproteinase 1,MMP-1 ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬基質金屬蛋白酶1(MMP-1)酶聯免疫試劑盒（CSB-E14057c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP-1含量。MMP-1，又稱間質膠原酶和成纖維細胞膠原酶，是基質金屬蛋白酶家族的一員，主要參與細胞外基質的降解。其在正常生理過程中如胚胎發育和組織重塑中起作用，同時在疾病過程中如癌癥和骨關節炎中，MMP-1的表達和活性增加，導致細胞外基質破壞，促進疾病進展。試劑盒檢測范圍為0.18 ng/mL-12 ng/mL，適用于科研場景中探究犬類疾病模型（如關節炎、皮膚損傷修復或腫瘤微環境）中MMP-1的動態變化。可為分子機制研究、藥物開發或生物標志物篩選提供定量數據支持，滿足科研工作者對犬類MMP-1活性監測的需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MMP-1
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.18 ng/mL-12 ng/mL
靈敏度：

0.04 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-102
1:2	Average %	89
	Range %	85-99
1:4	Average %	94
	Range %	85-97
1:8	Average %	90
	Range %	83-98

回收率：

The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	82-98
EDTA plasma (n=4)	97	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.